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Figure 3. P-selectin upregulation is reduced in LAT-/- platelets and absent in SLP-76-/- platelets. Washed murine platelets were stimulated with 0.5 U/ml thrombin, 40 nM Cvx, 10 nM Cvx, 20 µg/ml CRP, or 10 µg/ml CRP for 10 min at 37°C in the presence of FITC- -P-selectin followed by fixation and flow cytometric analysis. Results were then plotted as the percent of platelets staining positive for P-selectin.
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