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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.20072619
Weber et al.

Figure S1 Intracellular calcium flux in WT DT40 B cells.

Figure S2 Calcium flux in PLCγ2-GFP knock-in B cells.

Figure S3 Spreading and contraction of various DT40 B cells.

Video 1 Spreading, contraction, and antigen accumulation in WT DT40 B cells.

Video 2 Recruitment of GFP-Syk to antigen microclusters in WT DT40 B cells.

Video 3 Migratory behavior of Syk-KO DT40 B cells in response to membrane-bound antigen stimulation.

Video 4 Impaired spreading response and antigen accumulation in PLCγ2-KO DT40 B cells.

Video 5 Recruitment of PLCγ2-GFP to antigen microclusters during spreading and contraction in primary splenic B cells from the knock-in mouse.

Video 6 Recruitment of PLCγ2-GFP to antigen microclusters during spreading and contraction in primary splenic B cells from the knock-in mouse.

Video 7 Recruitment of PLCγ2-GFP to antigen microclusters in PLCγ2-KO–reconstituted DT40 B cells.

Video 8 Impaired spreading response in Blnk-KO DT40 B cells.

Video 9 Defective spreading response in Vav3-KO DT40 B cells.

 





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