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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.20060710
Mrass et al.

• Figure S1 TILs reveal an activated phenotype in both vaccinated and nonvaccinated tumors.

• Figure S2 Cognate antigen at the target site modifies the phenotype of TILs.

• Figure S3 Migratory parameters of nonconfined T cells within explanted EL4 and E.G7-OVA tumors.

• Figure S4 TC-1 ECFP tumors show similar growth characteristics and response to vaccination as parental TC-1 tumors.

• Table S1 Migratory properties of DPE^GFPxP14 T cells in EL4 and E.G7-OVA tumors in mice co-transferred with unlabeled OT-I T cells

• Table S2 Migratory properties of cotransferred OT-I-ECFP and P14-YFP cells in EL4 tumors

• Table S3 Migratory properties of cotransferred OT-I-ECFP and P14-YFP cells in E.G7-OVA tumors

• Video 1 Naive T cell migration within peripheral lymph nodes.

• Video 2 Naive T cell migration within peripheral lymph nodes.

• Video 3 TIL behavior in TC-1 tumors in nonvaccinated mice.

• Video 4 TIL behavior in TC-1 tumors 2 d after the vaccine boost.

• Video 5 TIL behavior in TC-1 tumors 4 d after the vaccine boost.

• Video 6 Migrating TILs reveal a polarized morphology and crawl along fibers of the extracellular matrix.

• Video 7 TILs engage in short- and long-term interactions with tumor cells.

• Video 8 A T cell engaging in long-term interaction with a tumor cell.

• Video 9 Apoptotic death of an individual tumor cell.

• Video 10 A DPE^GFPxOT-I effector CTLs stably interacting with an E.G7-OVA tumor cell.

• Video 11DPE^GFPxOT-I effector CTLs engage in sequential intermediate-length interactions with E.G7-OVA tumor cells.

• Video 12 DPE^GFPxOT-I effector CTLs engage in short-term interactions with EL4 tumor cells.

• Video 13 Covisualization of P14-YFP and OT-I-ECFP cells in E.G7-OVA tumor 3 d after adoptive transfer.

• Video 14 Covisualization of P14 and OT-I cells in E.G7-OVA tumor 4 d after adoptive transfer.

• Video 15 A migrating DPE^GFPxOT-I effector CTL initiating firm contact with a tissue macrophage.

• Video 16 In vivo imaging of DPE^GFPxOT-I effector CTLs within the EL4 tumor.

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