STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES: IV. VIRAL MULTIPLICATION IN A STABLE STRAIN OF HUMAN MALIGNANT EPITHELIAL CELLS (STRAIN HELA) DERIVED FROM AN EPIDERMOID CARCINOMA OF THE CERVIX

doi:10.1084/jem.97.5.695

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Scherer, W. F.
	Articles by Gey, G. O.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Scherer, W. F.
	Articles by Gey, G. O.


Social Bookmarking

	What's this?

The Journal of Experimental Medicine, Vol 97, 695-710, Copyright, 1953, by The Rockefeller Institute for Medical Research New York

ARTICLE

STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES : IV. VIRAL MULTIPLICATION IN A STABLE STRAIN OF HUMAN MALIGNANT EPITHELIAL CELLS (STRAIN HELA) DERIVED FROM AN EPIDERMOID CARCINOMA OF THE CERVIX

William F. Scherer M.D.¹, Jerome T. Syverton M.D.¹, and George O. Gey M.D.¹

¹ From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis, and the Department of Surgery, Johns Hopkins Hospital and University, Baltimore

The cells of a human epithelial cancer cultivated en massehave been shown to support the multiplication of all three typesof poliomyelitis virus. These cells (strain HeLa of Gey) havebeen maintained in vitro since their derivation from an epidermoidcarcinoma of the cervix in February, 1951. As the virus multipliedit caused in from 12 to 96 hours degeneration and destructionof the cancer cells. The specific destructive effect of thevirus was prevented by adding homotypic antibody to the culturesbut not by adding heterotypic antibodies.

Methods for the preparationof large numbers of replicate cultures with suspensions of strainHeLa cells were described. The cells in suspension were readilyquantitated by direct counts in a hemocytometer. A syntheticsolution that maintains cellular viability was employed forviral propagation.

The experimental results demonstrate theusefulness of strain HeLa cells for (a) the quantitation ofpoliomyelitis virus, (b) the measurement of poliomyelitis antibodies,and (c) the production of virus.

Submitted on January 19, 1953

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Miller, A. D., Bergholz, U., Ziegler, M., Stocking, C. (2008). Identification of the Myelin Protein Plasmolipin as the Cell Entry Receptor for Mus caroli Endogenous Retrovirus. J. Virol. 82: 6862-6868 [Abstract] [Full Text]
Nilsson, E. C., Jamshidi, F., Johansson, S. M. C., Oberste, M. S., Arnberg, N. (2008). Sialic Acid Is a Cellular Receptor for Coxsackievirus A24 Variant, an Emerging Virus with Pandemic Potential. J. Virol. 82: 3061-3068 [Abstract] [Full Text]
Fenner, B. J., Goh, W., Kwang, J. (2007). Dissection of Double-Stranded RNA Binding Protein B2 from Betanodavirus. J. Virol. 81: 5449-5459 [Abstract] [Full Text]
Yoshikawa, T., Iwasaki, T., Ida-Hosonuma, M., Yoneyama, M., Fujita, T., Horie, H., Miyazawa, M., Abe, S., Simizu, B., Koike, S. (2006). Role of the Alpha/Beta Interferon Response in the Acquisition of Susceptibility to Poliovirus by Kidney Cells in Culture. J. Virol. 80: 4313-4325 [Abstract] [Full Text]
Jogler, C., Hoffmann, D., Theegarten, D., Grunwald, T., Uberla, K., Wildner, O. (2006). Replication properties of human adenovirus in vivo and in cultures of primary cells from different animal species.. J. Virol. 80: 3549-3558 [Abstract] [Full Text]
Saha, M. N., Tanaka, A., Jinno-Oue, A., Shimizu, N., Tamura, K., Shinagawa, M., Chiba, J., Hoshino, H. (2005). Formation of Vesicular Stomatitis Virus Pseudotypes Bearing Surface Proteins of Hepatitis B Virus. J. Virol. 79: 12566-12574 [Abstract] [Full Text]
Ludemann, N., Clement, A., Hans, V. H., Leschik, J., Behl, C., Brandt, R. (2005). O-Glycosylation of the Tail Domain of Neurofilament Protein M in Human Neurons and in Spinal Cord Tissue of a Rat Model of Amyotrophic Lateral Sclerosis (ALS). J. Biol. Chem. 280: 31648-31658 [Abstract] [Full Text]
Guidez, F., Howell, L., Isalan, M., Cebrat, M., Alani, R. M., Ivins, S., Hormaeche, I., McConnell, M. J., Pierce, S., Cole, P. A., Licht, J., Zelent, A. (2005). Histone Acetyltransferase Activity of p300 Is Required for Transcriptional Repression by the Promyelocytic Leukemia Zinc Finger Protein. Mol. Cell. Biol. 25: 5552-5566 [Abstract] [Full Text]
Seelenmeyer, C., Wegehingel, S., Lechner, J., Nickel, W. (2003). The cancer antigen CA125 represents a novel counter receptor for galectin-1. J. Cell Sci. 116: 1305-1318 [Abstract] [Full Text]
Logg, C. R., Logg, A., Matusik, R. J., Bochner, B. H., Kasahara, N. (2002). Tissue-Specific Transcriptional Targeting of a Replication-Competent Retroviral Vector. J. Virol. 76: 12783-12791 [Abstract] [Full Text]
Wildner, O., Morris, J. C. (2000). The Role of the E1B 55 kDa Gene Product in Oncolytic Adenoviral Vectors Expressing Herpes Simplex Virus-tk: Assessment of Antitumor Efficacy and Toxicity. Cancer Res. 60: 4167-4174 [Abstract] [Full Text]
Gorry, P. R., Howard, J. L., Churchill, M. J., Anderson, J. L., Cunningham, A., Adrian, D., McPhee, D. A., Purcell, D. F.�J. (1999). Diminished Production of Human Immunodeficiency Virus Type 1�in Astrocytes Results from Inefficient Translation of gag, env, and nef mRNAs despite Efficient Expression of Tat and Rev. J. Virol. 73: 352-361 [Abstract] [Full Text]
Inoue, N., Russell, D. W. (1998). Packaging Cells Based on Inducible Gene Amplification for the Production of Adeno-Associated Virus Vectors. J. Virol. 72: 7024-7031 [Abstract] [Full Text]
Yamamoto, M, Ko, L J, Leonard, M W, Beug, H, Orkin, S H, Engel, J D (1990). Activity and tissue-specific expression of the transcription factor NF-E1 multigene family.. Genes Dev. 4: 1650-1662 [Abstract]
DEINHARDT, F., MAY, R. D., CALHOUN, H. H., SULLIVAN, H. E. (1958). The Isolation of Adenovirus Type 1 from a Fatal Case of Viral "Pneumonitis". Arch Intern Med 102: 816-819 [Abstract]
DREW, R. M. (1957). Isolation and Propagation of Rabbit Kidney Epitheliallike Cells. Science 126: 747-748
VAN HORNE, R. G., SASLAW, S., ANDERSON, G. R., FLATLEY, F. J., CARR, R. D. (1957). An Intrafamilial Epidemic of Pharyngoconjunctival Fever. Arch Intern Med 99: 70-73 [Abstract]
Eagle, H. (1955). Nutrition Needs of Mammalian Cells in Tissue Culture. Science 122: 501-504
Syverton, J. T., Scherer, W. F. (1953). APPLICATIONS OF STRAINS OF MAMMALIAN CELLS TO THE STUDY OF ANIMAL VIRUSES. Cold Spring Harb Symp Quant Biol 18: 285-289 [Abstract]