The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 91, 341-349, Copyright, 1950, by The Rockefeller Institute for Medical Research New York

ARTICLE

IMPROVED METHODS FOR THE PREPARATION OF THE SPECIFIC POLYSACCHARIDES OF PNEUMOCOCCUS

Michael Heidelberger Ph.D.1, Colin M. MacLeod M.D.1, Harold Markowitz 1, and Amy S. Roe 1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, the Presbyterian Hospital, and the Department of Microbiology, New York University College of Medicine, New York

The specific polysaccharides of Types I and V pneumococcus give sharp equivalence zones and show maximal precipitation with homologous rabbit antisera only when carefully prepared from cultures which have not been neutralized with alkali.

The precipitating power of S V toward homologous rabbit antiserum falls off in 0.1 N NaOH even more rapidly than that of S I, dropping to 7 per cent of the original value in 6 days at room temperature. The alkaline solution develops a large absorption peak at 270 mµ.

Directions are given for the preparation of S VII and S XII.

 Submitted on December 12, 1949


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