LOCALIZATION OF ANTIGEN IN TISSUE CELLS: II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY

doi:10.1084/jem.91.1.1

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The Journal of Experimental Medicine, Vol 91, 1-13, Copyright, 1950, by The Rockefeller Institute for Medical Research New York

ARTICLE

LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY

Albert H. Coons M.D.¹ and Melvin H. Kaplan ¹

¹ From the Department of Bacteriology and Immunology, Harvard Medical School, Boston

Improvements in a method for the specific microscopic localizationof antigen in tissue cells are described. This method employsantibody labelled with fluorescein isocyanate as a histochemicalstain, the specific antigen-antibody precipitate being madevisible under the fluorescence microscope.

Two isomeric seriesderived from nitrofluorescein are described.

Submitted on August 5, 1949

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