The Journal of Experimental Medicine
Avanti Polar Lipids
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The Journal of Experimental Medicine, Vol 87, 275-282, Copyright, 1948, by The Rockefeller Institute for Medical Research New York

ARTICLE

CHEMICAL STUDIES IN HOST-VIRUS INTERACTIONS : V. SOME ADDITIONAL METHODS OF DETERMINING NUTRITIONAL REQUIREMENTS FOR VIRUS MULTIPLICATION



Seymour S. Cohen Ph.D.1 and Catherine B. Fowler 1

1 From The Children's Hospital of Philadelphia (Department of Pediatrics), and the Department of Physiological Chemistry, University of Pennsylvania, School of Medicine, Philadelphia

Omission of a single constituent from a chemically defined medium approximating the virus growth-promoting properties of broth affects virus production in infected bacteria. This may be estimated by the one-step growth technique and the course of desoxyribose nucleic acid synthesis. Nine amino acids and one purine have been shown to be important by these tests. A combination of all constituents observed to be important by the single supplement and single omission techniques has approximated the virus growth-promoting properties of broth. Certain anomalous results have been commented upon.

Submitted on August 29, 1947


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