The Journal of Experimental Medicine, Vol 85, 381-394,
Copyright, 1947, by The Rockefeller Institute for Medical Research New York
A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES OF THIS ENZYME
Paul C. Zamecnik M.D.1,
Lydia E. Brewster 1, and
Fritz Lipmann M.D.1
1 From the Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, and the Biochemical Research Laboratory, Massachusetts General Hospital, Boston
A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.
Submitted on December 3, 1947