The Journal of Experimental Medicine
Fluorescence In Vivo Endomicroscopy
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The Journal of Experimental Medicine, Vol 85, 381-394, Copyright, 1947, by The Rockefeller Institute for Medical Research New York


ARTICLE

A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES OF THIS ENZYME

Paul C. Zamecnik M.D.1, Lydia E. Brewster 1, and Fritz Lipmann M.D.1

1 From the Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, and the Biochemical Research Laboratory, Massachusetts General Hospital, Boston

A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.

Submitted on December 3, 1947


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