FACTORS AFFECTING THE GROWTH OF TUBERCLE BACILLI IN LIQUID MEDIA

doi:10.1084/jem.83.5.409

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The Journal of Experimental Medicine, Vol 83, 409-423, Copyright, 1946, by The Rockefeller Institute for Medical Research New York

ARTICLE

FACTORS AFFECTING THE GROWTH OF TUBERCLE BACILLI IN LIQUID MEDIA

René J. Dubos Ph.D.¹ and Bernard D. Davis M.D.¹

¹ From the Laboratories of The Rockefeller Institute for Medical Research

1. Certain water-soluble esters of long chain fatty acids (inparticular of oleic acid) favor submerged and diffuse growthof mycobacteria throughout the depth of synthetic liquid media.

2.Esters of oleic acid increase considerably the amount of growthyielded by avian strains in synthetic media.

3. The additionof serum albumin to synthetic liquid media permits visible growthof minimal inocula of virulent human tubercle bacilli (10^–8mg.) within 11 to 15 days.

4. Cultures growing diffusely inmedia containing the water-soluble esters— with or withoutalbumin—consist of cells of classical morphology and stainingproperties, which again exhibit the usual mode of growth whenreturned to the standard synthetic or egg yolk media.

Submitted on February 20, 1946

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