STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES: III. AN IMPROVED METHOD FOR THE ISOLATION OF THE TRANSFORMING SUBSTANCE AND ITS APPLICATION TO PNEUMOCOCCUS TYPES II, III, AND VI

doi:10.1084/jem.83.2.97

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The Journal of Experimental Medicine, Vol 83, 97-104, Copyright, 1946, by The Rockefeller Institute for Medical Research New York

ARTICLE

STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES : III. AN IMPROVED METHOD FOR THE ISOLATION OF THE TRANSFORMING SUBSTANCE AND ITS APPLICATION TO PNEUMOCOCCUS TYPES II, III, AND VI

Maclyn McCarty M.D., Lieutenant Commander¹ and Oswald T. Avery M.D.¹

¹ From the United States Navy Research Unit at the Hospital of The Rockefeller Institute for Medical Research

1. An improved method is outlined for the isolation and purificationof the pneumococcal transforming substance. This method makesuse of the fact that citrate inhibits the destructive actionof the enzyme, desoxyribonuclease, which is released togetherwith the active material during lysis of the living bacterialcells. A fivefold greater yield of purified transforming agentis obtained by the present method than by the procedure previousiydescribed.

2. The specific transforming substance has been isolatedfrom pneumococci of types II and VI, in addition to Type III.In each instance the biologically active material has been foundto consist of desoxyribonucleic acid.

Submitted on October 9, 1945

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