The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 82, 207-215, Copyright, 1945, by The Rockefeller Institute for Medical Research New York

ARTICLE

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD



Elvin A. Kabat Ph.D.1 and Ada E. Bezer 1

1 From the Department of Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute of New York

1. With purified blood group A and B specific substances from the gastric mucin of pigs precipitin reactions can be obtained in sera containing homologous isoagglutinins. By microquantitative precipitin technics the anti-A and anti-B content of normal and immune isoagglutinin-containing sera can be estimated on a weight basis.

2. There was a general correlation between antibody N precipitable from the sera of groups O and B by A substance and the agglutinin titer for A erythrocytes before and after immunization with blood group substances.

3. The reaction between A substance and its homologous isoagglutinin present in the sera of immunized individuals was of the same type as in other antigen-antibody systems.

 Submitted on June 5, 1945


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