The Journal of Experimental Medicine
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 908K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bier, O. G.
Right arrow Articles by Heidelberger, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Bier, O. G.
Right arrow Articles by Heidelberger, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 81, 449-468, Copyright, 1945, by The Rockefeller Institute for Medical Research New York

ARTICLE

A COMPARISON OF HUMAN AND GUINEA PIG COMPLEMENTS AND THEIR COMPONENT FRACTIONS

Otto G. Bier M.D.1, Graciela Leyton 1, Manfred M. Mayer 1, and Michael Heidelberger Ph.D.1

1 From the Departments of Medicine and Biochemistry, Columbia University, College of Physicians and Surgeons, and the Presbyterian Hospital, New York

1. Defects in methods previously proposed for the estimation of complement components are: failure to ensure an excess of the desired components and failure to ensure absence of anticomplementary effects in the dilution ranges used. Existing data are therefore subject to these uncertainties.

2. Methods are proposed for controlling the adequacy of the reagent for each component, for using it in dilutions below its anticomplementary range, and for reinforcing it with necessary components if these are present in inadequate amounts.

3. Titrations are given of the four components in human and guinea pig complements and in "midpiece," "endpiece," and in the various reagents used, including one with C'3 reactivity, solely, and one withC'4 reactivity.

4. C'3 is shown to be the component which usually limits the titer in guinea pig complement, and C'2 the component of lowest titer in human complement.

5. In immune hemolysis, each component of human complement may be replaced by the corresponding component of guinea pig complement and vice versa.

 Submitted on February 6, 1945


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS