The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katz, Y. J.
Right arrow Articles by Goldblatt, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Katz, Y. J.
Right arrow Articles by Goldblatt, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 78, 67-74, Copyright, 1943, by The Rockefeller Institute for Medical Research New York

ARTICLE

STUDIES ON EXPERIMENTAL HYPERTENSION : XXI. THE PURIFICATION OF RENIN



Yale J. Katz Ph.D.1 and Harry Goldblatt M.D.1

1 From the Institute of Pathology, Western Reserve University, Cleveland

1. Extraction of finely ground fresh hog kidney with distilled water adjusted to pH 7.8 with sodium hydroxide, followed by successive treatment, as described, with trichloroacetic acid and acetone, gives renin in good yield of a purity suitable for physiological studies and a good starting material for further purification. It contains 15 dog units per mg. N.

2. Further successive purification of this material with ethyl alcohol and ammonium sulfate gives a preparation containing 130 dog units per mg. N. The purest preparation hitherto reported (15) contained 16.0 to 20.8 units per mg. N. Preliminary Tiselius electrophoresis studies suggest homogeneity, but further studies to establish purity are in progress.

3. The properties of the most purified renin indicate that it is a protein. Its chemical and physiological properties correspond to those of the material in crude renal extract which induces an elevation of blood pressure when it is injected intravenously.

 Submitted on May 1, 1943


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS