The Journal of Experimental Medicine, Vol 78, 17-26,
Copyright, 1943, by The Rockefeller Institute for Medical Research New York
THE INFLUENCE OF AGE OF HOST AND TEMPERATURE OF INCUBATION ON INFECTION OF THE CHICK EMBRYO WITH VESICULAR STOMATITIS VIRUS
Björn Sigurdsson M.D.1
1 From the Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, New Jersey
Chick embryos after 7 days of incubation were found to be much more susceptible to infection with vesicular stomatitis virus than were 10 day embryos. They had a 100 per cent mortality and were very suitable for titrations of the virus. The rate of increase of virus in 7 and 10 day embryos was studied. Two different temperatures of incubation were employed, 35–36°C. and 39–40°C., and the growth curves for the virus under the different conditions are presented. 10 day embryos were highly resistant and at 39–40°C. more than half of them survived. At the lower temperature of incubation, 35–36°C., all 10 day embryos died, but they survived much longer than did 7 day embryos.
In the 7 day embryos death occurred after about 12 hours at 39–40°C. and after about 16 hours at 35–36°C., or earlier at the higher temperature.
In embryos of both ages the virus titer reached at the higher temperature was only about 1 per cent of that reached at 35–36°C., even in those that died.
Submitted on April 10, 1943
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