The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 77, 155-164, Copyright, 1943, by The Rockefeller Institute for Medical Research New York

ARTICLE

THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS



Theodore Shedlovsky Ph.D.1, Alexandre Rothen Ph.D.1, and Joseph E. Smadel M.D.1

1 From the Laboratories and the Hospital of The Rockefeller Institute for Medical Research

Studies on LS-protein, the soluble double antigen of vaccinia, and on the degradation products L'S and L''S' have been made with electrophoresis and in the analytical ultracentrifuge.

LS, which is homogeneous electrically and in the ultracentrifuge, has an isoelectric point at pH 4.8. At 4°C. its partial specific volume is 0.72 cc./gm., and its diffusion constant is 1.50 x 10–7 cm.2/sec. The sedimentation constant is 6.35 at 20°C., the molecular weight is 214,000, and the molecule appears to have an elongated ellipsoidal shape with an axis ratio of 1:20.

L'S and L''S' are homogeneous electrically but not in the ultracentrifuge, L''S' being extremely polydisperse.

 Submitted on November 13, 1942


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