The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 76, 211-220, Copyright, 1942, by The Rockefeller Institute for Medical Research New York

ARTICLE

THE MANUFACTURE OF ANTIBODIES IN VITRO

Linus Pauling Ph.D.1 and Dan H. Campbell Ph.D.1

1 From the Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena, California

A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine gamma-globulin and the dye with alkali and then slowly neutralizing the alkali. Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures.

By heating solutions of gamma-globulin and antigen to 57°C. for several days antisera homologous to the antigens have been prepared. This method has been used successfully with the azodye 1,3-dihydroxy-2,4,6-tri(p-azophenyl-arsonic acid) benzene and with pneumococcus polysaccharide Type III. The antipneumococcus sera were found to precipitate the polysaccharide of Type III but not those of Types I and VIII and to agglutinate pneumococci of Type III but not those of Types I and II.

 Submitted on April 25, 1942


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