The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 75, 285-295, Copyright, 1942, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN : IV. ADDITION OF HUMAN COMPLEMENT TO SPECIFIC PRECIPITATES



Michael Heidelberger Ph.D.1 and Manfred Mayer 1

1 From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. A modified method is given for the titration of human complement so that C'1 titers are measured, as in guinea pig serum, instead of the C'2 titers yielded by the usual titration.

2. The measurement of complement combining component or components in weight units, instead of relative terms, is carried out as in guinea pig serum and leads to similar values, 0.03 to 0.05 mg. of C' N per ml. of human serum.

3. Other similarities in human and guinea pig complements are noted and discussed.

 Submitted on December 12, 1941


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