The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 74, 359-367, Copyright, 1941, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN : III. UPTAKE OF COMPLEMENT NITROGEN UNDER VARYING EXPERIMENTAL CONDITIONS



Michael Heidelberger Ph.D.1, M. Rocha e Silva M.D.1, and Manfred Mayer 1

1 From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. Quantitative data are given on the effect of variations in the time of contact and the proportions of the reactants on the quantity of complement combining component nitrogen (C'1 N) found in active guinea pig serum.

2. C'1 N was the same when determined with precipitates containing excess antibody or excess antigen.

3. Finely divided specific precipitates took up the complement combining component (C'1) from subsequently added guinea pig serum almost as well as specific precipitates formed in the presence of complement.

 Submitted on June 26, 1941


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