The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 73, 695-709, Copyright, 1941, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN : II. THE INTERRELATION OF COMPLEMENT WITH ANTIGEN-ANTIBODY COMPOUNDS AND WITH SENSITIZED RED CELLS



Michael Heidelberger Ph.D.1, Alfred J. Weil M.D.1, and Henry P. Treffers Ph.D.1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. The molecular quantities of hemolysin and complement combining component or components (C'1) involved in hemolysis have been calculated on the basis of new, quantitative, absolute methods of analysis.

2. Molecular combining ratios between antigen, antibody, and C'1 have been established.

3. The data are shown to be in accord with the theory of combination of multivalent antigen with multivalent antibody.

4. The fixation of complement by antigen-antibody combination is qualitatively and quantitatively accounted for on this basis.

 Submitted on March 21, 1941


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