The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 72, 685-696, Copyright, 1940, by The Rockefeller Institute for Medical Research New York

ARTICLE

CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : III. THE EFFECT OF PURIFIED ENZYMES ON ELEMENTARY BODIES OF VACCINIA



Charles L. Hoagland M.D.1, S. M. Ward 1, Joseph E. Smadel M.D.1, and Thomas M. Rivers M.D.1

1 From the Hospital of The Rockefeller Institute for Medical Research

The effects of a number of crystalline and highly purified enzymes on elementary bodies of vaccinia are reported. These effects have been followed by determination of amino nitrogen, staining reaction, and studies of infectivity.

Pepsin, at a pH which inactivates the virus, results in its solution and rapid release of amino nitrogen.

Crystalline trypsin, chymotrypsin, carboxypeptidase, and ribonuclease are without appreciable effect on the virus.

Papain within a short time produces profound alteration in the staining reaction of the elementary body with release of amino nitrogen accompanied by complete inactivation of the virus. This reaction is not shared by crystalline ficin, another plant papain, or by cathepsin, an intracellular proteinase analogous to plant papains but of animal origin.

 Submitted on August 30, 1940


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