The Journal of Experimental Medicine
PBL InterferonSource
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 706K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Turner, T. B.
Right arrow Articles by Brayton, N. L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Turner, T. B.
Right arrow Articles by Brayton, N. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 70, 639-650, Copyright, 1939, by The Rockefeller Institute for Medical Research New York

ARTICLE

FACTORS INFLUENCING THE SURVIVAL OF SPIROCHETES IN THE FROZEN STATE

Thomas B. Turner M.D.1 and Nancy L. Brayton 1

1 From the Department of Bacteriology of The Johns Hopkins School of Hygiene and Public Health, Baltimore

Titration experiments made with relapsing fever spirochetes before and after freezing showed the following:

1. With each freezing and thawing there is a slight but regular decrease in virulence, which decrease bears no relation to the duration of storage at – 78°C. Ordinarily infectivity is destroyed by more than 4 refreezings.

2. There was not always close correlation between motility and infectivity.

3. Cooling spirochetes from 0°C. to –78°C. over a 2 to 6 hour period damages them only slightly more than does rapid cooling, but warming from – 78°C. to 0°C. over a 2 to 6 hour period kills most of the organisms. Rapid thawing, as in a water bath, damages the spirochetes less than thawing more slowly, as at room temperature.

4. At storage temperatures of –12°C. and –20°C. there is a gradual decrease in virulence over a period of days or weeks, and by the 6th week the infectivity of the material is markedly reduced.

 Submitted on October 4, 1939


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS