The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 68, 1-16, Copyright, 1938, by The Rockefeller Institute for Medical Research New York

ARTICLE

HORMONE STUDIES WITH THE ULTRACENTRIFUGE : I. AN IMPROVED AIR-DRIVEN VACUUM ULTRACENTRIFUGE SUITABLE FOR CONCENTRATION WORK IN BIOLOGICAL EXPERIMENTS



James A. Chiles Jr. Ph.D.1 and Aura E. Severinghaus Ph.D1

1 From the Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York

1. An ultracentrifuge is described in which the rotor is driven by a compressed air turbine, and is spun in an evacuated chamber to minimize friction and heating. The rotating parts are supported by a cushion of air in an air bearing.

2. The centrifuge rotor holds 10 test tubes inclined at 45° to the axis, and has a capacity of 55 cc. It is operated at a maximum speed of 51,000 R.P.M., which develops at the top of the fluid column in the test tubes a centrifugal field of over 100,000 times gravity, and at the bottom of the fluid column a field of over 200,000 times gravity.

3. By means of a reverse turbine, the rotor can be brought to a stop from full speed in a relatively short time.

4. A precession damping device is described, which effectively damps the precession and wobbling of the rotor that usually occurs at certain speeds in machines of this type.

5. A relatively long section of shaft is used between the centrifuge rotor and lower bearings. This prevents vibrations from being appreciably transmitted through the shaft to the lower bearings and driving mechanism, and results in a negligible wear on the bearings.

6. The driving mechanism is designed so that the positions of its parts are adjustable, and so that the driving mechanism may be dismantled without disturbing these adjustments.

 Submitted on April 4, 1938


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