The Journal of Experimental Medicine
Janeway's Immunobiology 7th Edition
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The Journal of Experimental Medicine, Vol 63, 819-826, Copyright, 1936, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : EFFECT OF SALTS ON THE REACTION



Michael Heidelberger Ph.D.1, Forrest E. Kendall Ph.D.1, and Torsten Teorell M.D.1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. A quantitative study has been made of the effect on the precipitin reaction between the specific polysaccharide of Type III pneumococcus and the homologous antibody of salt concentrations ranging from O.1 M to 1.79 M, including the effect of ions of higher valence.

2. Within these limits, observed decreases in precipitated antibody with increasing salt concentration appear to be due to a decrease in the amount of antibody combined with the S III, rather than to an increase in solubility of the S III-antibody compounds.

3. The egg albumin-antibody reaction is far less sensitive to changes in salt concentration than is the S III-antibody reaction.

 Submitted on March 19, 1936


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