The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 62, 467-483, Copyright, 1935, by The Rockefeller Institute for Medical Research New York

ARTICLE

A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : II. A STUDY OF AN AZOPROTEIN-ANTIBODY SYSTEM



Michael Heidelberger Ph.D.1 and Forrest E. Kendall Ph.D.1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. A quantitative theory of the precipitin reaction based on the laws of classical chemistry has been tested on an azoprotein-antiprotein system and found to apply.

2. With its aid relationships may be deduced which permit the calculation of the behavior of an antidye serum over its entire range after a few quantitative chemical analyses have been made for antigen and antibody in the precipitate.

3. An empirical relation is also presented which further reduces the number of analyses necessary.

4. A study of supernatants in the inhibition zone has shown that the entire amount of dissolved antigen-antibody compound present is precipitated when supernatants are analyzed for antigen by the precipitin method.

 Submitted on June 23, 1935


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