The Journal of Experimental Medicine
ThymUS '08
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The Journal of Experimental Medicine, Vol 56, 91-106, Copyright, 1932, by The Rockefeller Institute for Medical Research New York

ARTICLE

PROPERTIES OF THE CAUSATIVE AGENT OF A CHICKEN TUMOR : III. ATTEMPTS AT ISOLATION OF THE ACTIVE PRINCIPLE



James B. Murphy M.D.1, Ernest Sturm 1, Albert Claude M.D.1, and Oscar M. Helmer Ph.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research

By two methods a protein fraction can be separated out from a Chicken Tumor I extract, which carries all the tumor-producing agent. The precipitate can be dissolved and reprecipitated a number of times without loss of activity. The agent can be largely dissociated from the protein as shown by the fact that aluminum hydroxide will adsorb the protein from an extract and leave the agent behind. This purified material has a very low protein content, if any, as shown by both chemical and biological tests.

Submitted on March 11, 1932


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