The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 55, 555-561, Copyright, 1932, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE



Michael Heidelberger Ph.D.1 and Forrest E. Kendall Ph.D.1

1 From the Department of Medicine, Presbyterian Hospital, and the College of Physicians and Surgeons, Columbia University, New York

A method, based on the precipitin reaction, is given for the micro determination of the specific polysaccharide of Type III pneumococcus. As little as 0.01 mg. of S III can be determined. The method should be applicable to any specific polysaccharide upon standardization of an homologous antibody solution or antiserum in the region of excess antibody.

Submitted on December 21, 1931


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