The Journal of Experimental Medicine
Avanti Polar Lipids
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The Journal of Experimental Medicine, Vol 52, 477-483, Copyright, 1930, by The Rockefeller Institute for Medical Research New York

ARTICLE

SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERA

Michael Heidelberger Ph.D.1, Richard H. P. Sia M.D.1, and Forrest E. Kendall Ph.D.1

1 From the Laboratories of the Department of Practice of Medicine, Presbyterian Hospital and College of Physicians and Surgeons, New York

1. A rapid and simple method is given for the approximate determination of the specifically precipitable protein in Type I antipneumococcus sera.

2. It is shown that a close parallel exists between the specifically precipitable protein and the number of mouse protection units in a wide variety of Type I antipneumococcus sera.

3. Owing to the consistent results obtained and the rapidity, simplicity, and economy of the method, its use is proposed instead of the mouse protection test as a basis for the titration of standard sera and the comparison of others with a standard.

4. A method is given for conveniently preparing highly purified specific polysaccharide of Type I pneumococcus.

 Submitted on June 16, 1930


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