The Journal of Experimental Medicine
PBL InterferonSource
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carrel, A.
Right arrow Articles by Ebeling, A. H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Carrel, A.
Right arrow Articles by Ebeling, A. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 37, 653-658, Copyright, 1923, by The Rockefeller Institute for Medical Research New York

ARTICLE

ANTAGONISTIC GROWTH-ACTIVATING AND GROWTH-INHIBITING PRINCIPLES IN SERUM

Alexis Carrel M.D.1 and Albert H. Ebeling M.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research.

The substances precipitated by CO2 from the serum of chickens 1 or 2 years old slightly increased the activity of pure cultures of a 10 year old strain of fibroblasts, an indication that a growth-promoting substance had been obtained from the serum. The rate of growth of fibroblasts was slower in serum deprived of its globulins and the increase of the inhibiting effect of the treated serum is thus manifested. It would appear that the inhibiting action of serum is referable to one or several growth-activating substances which precipitate by CO2, and to one or several growth-retarding substances which remain in the supernatant serum after the precipitate has been thrown down by centrifugation.

It may be concluded that, under the conditions of the experiments:

1. A substance enhancing the proliferative activity of homologous fibroblasts is precipitated from serum by CO2.

2. The serum from which the precipitate has been removed inhibits the proliferation of homologous fibroblasts more markedly than does the original serum. The solution of the precipitate in the treated serum has about the same effect on fibroblasts as the original serum.

3. The restraining effect of serum on the activity of homologous fibroblasts is due in part to the antagonistic action of growth-activating and inhibiting substances.

 Submitted on December 27, 1922


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS