The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 36, 365-377, Copyright, 1922, by The Rockefeller Institute for Medical Research New York

ARTICLE

PURE CULTURES OF LARGE MONONUCLEAR LEUCOCYTES

Alexis Carrel M.D.1 and Albert H. Ebeling M.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research.

1. Pure strains of mononuclear leucocytes were isolated from the blood of adult chickens and keptin active condition for nearly 3 months.

2. The cultures were composed of large mononuclear leucocytes which migrated and proliferated in vitro at a slower rate than fibroblasts. The cells had no tendency to form a tissue, as do fibroblasts and epithelial cells. They were much less resistant than fibroblasts.

3. Differentiation of the large mononuclears into cells assuming the appearance of fibroblasts took place under certain conditions.

4. The activity of the large mononuclears was increased by embryonic tissue juice and inhibited by homologous serum.

 Submitted on March 16, 1922


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