The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 36, 285-289, Copyright, 1922, by The Rockefeller Institute for Medical Research New York

ARTICLE

MIXED CULTURES OF PURE STRAINS OF FIBROBLASTS AND EPITHELIAL CELLS

Albert H. Ebeling M.D.1 and Albert Fischer M.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research.

1. Strains of epithelium and fibroblasts cultivated side by side in the same medium keep their individual characteristics. When sectioned and stained by the Van Gieson method, the cultures show the epithelium stained greenish yellow and the fibroblasts and their fibrillæ pink.

2. There are no transition forms between the epithelial cells and fibroblasts.

3. The epithelial cells belonging to an older strain are still able to form primitive structures of winding tubules, with typical glandular epithelium.

4. Under the conditions of the experiments, no dedifferentiation takes place.

 Submitted on May 4, 1922


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