The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 34, 317-337, Copyright, 1921, by The Rockefeller Institute for Medical Research New York

ARTICLE

THE MULTIPLICATION OF FIBROBLASTS IN VITRO

Alexis Carrel M.D.1 and Albert H. Ebeling M.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research.

1. It may be concluded that, under the conditions of the experiments and within the limits of accuracy of the method, the temporary multiplication of fibroblasts cultivated in the plasma of an adult animal is not due to the serum. It may be attributed to the presence of a small amount of embryonic juice within the tissue itself.

2. The indefinite multiplication of fibroblasts in a medium composed of adult plasma and of embryonic juice is due neither to the serum nor to the fibrin. It depends entirely on substances contained in the embryonic juice.

3. There is a definite relation between the rate of growth and the concentration of the embryonic juice in the medium.

 Submitted on April 13, 1921


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