The Journal of Experimental Medicine
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Published online June 2, 2008
doi:10.1084/jem.20071555
The Journal of Experimental Medicine, Vol. 205, No. 6, 1331-1342
The Rockefeller University Press, 0022-1007 $30.00
© 2008 Weller et al.
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ARTICLE

 Somatic diversification in the absence of antigen-driven responses is the hallmark of the IgM+IgD+CD27+ B cell repertoire in infants

Sandra Weller1, Maria Mamani-Matsuda1, Capucine Picard2,4, Corinne Cordier3, Damiana Lecoeuche1, Frédéric Gauthier5, Jean-Claude Weill1, and Claude-Agnès Reynaud1

1 Institut National de la Santé et de la Recherche Médicale (INSERM) U783, Développement du Système Immunitaire, 2 INSERM U550, Génétique des maladies infectieuses, 3 INSERM IFR 94, Service commun de Tri cellulaire, Université Paris Descartes, Faculté de Médecine, Site Necker Enfants-Malades, Paris 75015, France
4 Centre d'étude des déficits immunitaires, Hôpital Necker Enfants-Malades, Paris 75015, France
5 Service de Chirurgie Pédiatrique, Hôpital Bicêtre, Le Kremlin Bicêtre 94270, France

CORRESPONDENCE Jean-Claude Weill: weill{at}necker.fr OR Claude-Agnès Reynaud: reynaud{at}necker.fr

T cell–dependent immune responses develop soon after birth, whereas it takes 2 yr for humans to develop T cell–independent responses. We used this dissociation to analyze the repertoire diversification of IgM+IgD+CD27+ B cells (also known as "IgM memory" B cells), comparing these cells with switched B cells in children <2 yr of age, with the aim of determining whether these two subsets are developmentally related. We show that the repertoire of IgM+IgD+CD27+ B cells in the spleen and blood displays no sign of antigen-driven activation and expansion on H-CDR3 spectratyping, despite the many antigenic challenges provided by childhood vaccinations. This repertoire differed markedly from those of switched B cells and splenic germinal center B cells, even at the early stage of differentiation associated with µ heavy chain expression. These data provide evidence for the developmental diversification of IgM+IgD+CD27+ B cells, at least in very young children, outside of T cell–dependent and –independent immune responses.

Abbreviations used: AID, activation-induced cytidine deaminase; GC, germinal center; MZ, marginal zone; SMZ, splenic MZ; TD, T-dependent; TI, T cell–independent.


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