The Journal of Experimental Medicine
VISIT JCB ONLINE!
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online February 20, 2007
doi:10.1084/jem.20062508
The Journal of Experimental Medicine, Vol. 204, No. 3, 525-532
The Rockefeller University Press, 0022-1007 $30.00
© 2007 Tellam et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tellam, J.
Right arrow Articles by Khanna, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tellam, J.
Right arrow Articles by Khanna, R.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ARTICLE

 Influence of translation efficiency of homologous viral proteins on the endogenous presentation of CD8+ T cell epitopes

Judy Tellam1, Mark H. Fogg2, Michael Rist1, Geoff Connolly1, David Tscharke1, Natasha Webb1, Lea Heslop1, Fred Wang2, and Rajiv Khanna1

1 Australian Centre for Vaccine Development and Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Clive Berghofer Cancer Research Centre, Queensland Institute of Medical Research, Brisbane (Qld) 4006, Australia
2 Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115

CORRESPONDENCE Rajiv Khanna: rajivK{at}qimr.edu.au OR Judy Tellam: judyT{at}qimr.edu.au

A significant proportion of endogenously processed CD8+ T cell epitopes are derived from newly synthesized proteins and rapidly degrading polypeptides (RDPs). It has been hypothesized that the generation of rapidly degrading polypeptides and CD8+ T cell epitopes from these RDP precursors may be influenced by the efficiency of protein translation. Here we address this hypothesis by using the Epstein-Barr virus–encoded nuclear antigen 1 protein (EBNA1), with or without its internal glycine-alanine repeat sequence (EBNA1 and EBNA1{Delta}GA, respectively), which display distinct differences in translation efficiency. We demonstrate that RDPs constitute a significant proportion of newly synthesized EBNA1 and EBNA1{Delta}GA and that the levels of RDPs produced by each of these proteins directly correlate with the translation efficiency of either EBNA1 or EBNA1{Delta}GA. As a consequence, a higher number of major histocompatibility complex–peptide complexes can be detected on the surface of cells expressing EBNA1{Delta}GA, and these cells are more efficiently recognized by virus-specific cytotoxic T lymphocytes compared to the full-length EBNA1. More importantly, we also demonstrate that the endogenous processing of these CD8+ T cell epitopes is predominantly determined by the rate at which the RDPs are generated rather than the intracellular turnover of these proteins.

Abbreviations used: DRiP, defective ribosomal product; EBNA1, EBV-encoded nuclear antigen 1 protein; LCL, lymphoblastoid cell line; RDP, rapidly degrading polypeptide.

J. Tellam and M.H. Fogg contributed equally to this work.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Translated to death
Hema Bashyam
J. Exp. Med. 2007 204: 455b. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS