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Published online 3 July 2006 doi:10.1084/jem.20060468
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 7, 1693-1700
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BRIEF DEFINITIVE REPORT

 Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells

Nabila Seddiki1,2,3, Brigitte Santner-Nanan4, Jeff Martinson5, John Zaunders2, Sarah Sasson2, Alan Landay5, Michael Solomon6, Warwick Selby6, Stephen I. Alexander7, Ralph Nanan4, Anthony Kelleher2,3, and Barbara Fazekas de St. Groth1

1 Centenary Institute of Cancer Medicine and Cell Biology, Faculty of Medicine, University of Sydney, Newtown, NSW 2042, Australia
2 Centre for Immunology, St. Vincents Hospital, Darlinghurst, NSW 2010, Australia
3 National Centre in HIV Epidemiology and Clinical Research, University of NSW, Kensington, NSW 2052, Australia
4 Department of Paediatrics, University of Sydney, Western Clinical School, Penrith, NSW 2751, Australia
5 Department of Immunology/Microbiology, Rush University Medical Center, Chicago, IL 60612
6 Department of Gastroenterology, The Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia
7 The Children's Hospital at Westmead, NSW 2145, Australia

CORRESPONDENCE Barbara Fazekas de St. Groth: b.fazekas{at}centenary.usyd.edu.au

Abnormalities in CD4+CD25+Foxp3+ regulatory T (T reg) cells have been implicated in susceptibility to allergic, autoimmune, and immunoinflammatory conditions. However, phenotypic and functional assessment of human T reg cells has been hampered by difficulty in distinguishing between CD25-expressing activated and regulatory T cells. Here, we show that expression of CD127, the {alpha} chain of the interleukin-7 receptor, allows an unambiguous flow cytometry–based distinction to be made between CD127lo T reg cells and CD127hi conventional T cells within the CD25+CD45RO+RA– effector/memory and CD45RA+RO– naive compartments in peripheral blood and lymph node. In healthy volunteers, peripheral blood CD25+CD127lo cells comprised 6.35 ± 0.26% of CD4+ T cells, of which 2.05 ± 0.14% expressed the naive subset marker CD45RA. Expression of FoxP3 protein and the CD127lo phenotype were highly correlated within the CD4+CD25+ population. Moreover, both effector/memory and naive CD25+CD127lo cells manifested suppressive activity in vitro, whereas CD25+CD127hi cells did not. Cell surface expression of CD127 therefore allows accurate estimation of T reg cell numbers and isolation of pure populations for in vitro studies and should contribute to our understanding of regulatory abnormalities in immunopathic diseases.


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