Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Benitez-Ribas, D. Articles by de Vries, I. J. M. Search for Related Content PubMed PubMed Citation Articles by Benitez-Ribas, D. Articles by de Vries, I. J. M. Social Bookmarking                      What's this?

Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4⁺ T cells after FcRII-mediated uptake

¹ Department of Tumor Immunology, ² Department of Blood Transfusion and Transplantation Immunology, ³ Department of Medical Oncology, and ⁴ Department of Pediatric Oncology, Radboud University Nijmegen Medical Centre and Nijmegen Centre for Molecular Life Sciences, 6500 HB Nijmegen, Netherlands
⁵ Miltenyi Biotec GmbH, D-51429 Bergisch Gladbach, Germany

CORRESPONDENCE I. Jolanda M. de Vries: j.devries{at}ncmls.ru.nl

Plasmacytoid dendritic cells (pDCs) contribute to innate antiviral immune responses by producing type I interferons. Although human pDCs can induce T cell responses upon viral infection, it remains unclear if pDCs can present exogenous antigens. Here, we show that human pDCs exploit FcRII (CD32) to internalize antigen–antibody complexes, resulting in the presentation of exogenous antigen to T cells. pDCs isolated from melanoma patients vaccinated with autologous monocyte-derived peptide- and keyhold limpet hemocyanin (KLH)–loaded dendritic cells, but not from nonvaccinated patients or patients that lack a humoral response against KLH, were able to stimulate KLH-specific T cell proliferation. Interestingly, we observed that internalization of KLH by pDCs depended on the presence of serum from vaccinated patients that developed an anti-KLH antibody response. Anti-CD32 antibodies inhibited antigen uptake and presentation, demonstrating that circulating anti-KLH antibodies binding to CD32 mediate KLH internalization. We conclude that CD32 is an antigen uptake receptor on pDCs and that antigen presentation by pDCs is of particular relevance when circulating antibodies are present. Antigen presentation by pDCs may thus modulate the strength and quality of the secondary phase of an immune response.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

• Meyer-Wentrup, F., Benitez-Ribas, D., Tacken, P. J., Punt, C. J. A., Figdor, C. G., de Vries, I. J. M., Adema, G. J. (2008). Targeting DCIR on human plasmacytoid dendritic cells results in antigen presentation and inhibits IFN-{alpha} production. Blood 111: 4245-4253 [Abstract] [Full Text]  
• Sapoznikov, A., Fischer, J. A.A., Zaft, T., Krauthgamer, R., Dzionek, A., Jung, S. (2007). Organ-dependent in vivo priming of naive CD4+,but not CD8+,T cells by plasmacytoid dendritic cells. J. Exp. Med. 204: 1923-1933 [Abstract] [Full Text]  
• Faith, A., Peek, E., McDonald, J., Urry, Z., Richards, D. F., Tan, C., Santis, G., Hawrylowicz, C. (2007). Plasmacytoid Dendritic Cells from Human Lung Cancer Draining Lymph Nodes Induce Tc1 Responses. Am. J. Respir. Cell Mol. Bio. 36: 360-367 [Abstract] [Full Text]  
• Liu, Y., Gao, X., Masuda, E., Redecha, P. B., Blank, M. C., Pricop, L. (2006). Regulated Expression of Fc{gamma}R in Human Dendritic Cells Controls Cross-Presentation of Antigen-Antibody Complexes. J. Immunol. 177: 8440-8447 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS