The Journal of Experimental Medicine
CSHL Meetings & Courses
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 20 November 2006 doi:10.1084/jem.20060710
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 12, 2749-2761
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mrass, P.
Right arrow Articles by Weninger, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mrass, P.
Right arrow Articles by Weninger, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ARTICLE

 Random migration precedes stable target cell interactions of tumor-infiltrating T cells

Paulus Mrass1, Hajime Takano2, Lai Guan Ng1, Sachin Daxini1, Marcio O. Lasaro1, Amaya Iparraguirre1, Lois L. Cavanagh1, Ulrich H. von Andrian5, Hildegund C.J. Ertl1, Philip G. Haydon2, and Wolfgang Weninger1,3,4

1 Immunology Program, The Wistar Institute, Philadelphia, PA 19104
2 Department of Neuroscience and Conte Center for Integration at the Tripartite Synapse, 3 Department of Dermatology, and 4 Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
5 CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02215

CORRESPONDENCE Wolfgang Weninger: weninger{at}wistar.org

The tumor microenvironment is composed of an intricate mixture of tumor and host-derived cells that engage in a continuous interplay. T cells are particularly important in this context as they may recognize tumor-associated antigens and induce tumor regression. However, the precise identity of cells targeted by tumor-infiltrating T lymphocytes (TILs) as well as the kinetics and anatomy of TIL-target cell interactions within tumors are incompletely understood. Furthermore, the spatiotemporal conditions of TIL locomotion through the tumor stroma, as a prerequisite for establishing contact with target cells, have not been analyzed. These shortcomings limit the rational design of immunotherapeutic strategies that aim to overcome tumor-immune evasion. We have used two-photon microscopy to determine, in a dynamic manner, the requirements leading to tumor regression by TILs. Key observations were that TILs migrated randomly throughout the tumor microenvironment and that, in the absence of cognate antigen, they were incapable of sustaining active migration. Furthermore, TILs in regressing tumors formed long-lasting (≥30 min), cognate antigen–dependent contacts with tumor cells. Finally, TILs physically interacted with macrophages, suggesting tumor antigen cross-presentation by these cells. Our results demonstrate that recognition of cognate antigen within tumors is a critical determinant of optimal TIL migration and target cell interactions, and argue against TIL guidance by long-range chemokine gradients.

Abbreviations used: ECFP, enhanced cyan fluorescent protein; ECM, extracellular matrix; EYFP, enhanced yellow fluorescent protein; PLN, peripheral LN; TIL, tumor-infiltrating T lymphocyte.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS