Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gauduin, M.-C. Articles by Johnson, R. P. Search for Related Content PubMed PubMed Citation Articles by Gauduin, M.-C. Articles by Johnson, R. P. Social Bookmarking                      What's this?

¹ Division of Immunology, Division of ² Microbiology, and Division of ³ Primate Medicine, New England Primate Research Center, Harvard Medical School, Southborough, MA 01772
⁴ AIDS Vaccine Program, Science Applications International Corporation Frederick, Inc., National Cancer Institute (NCI)-Frederick, Frederick, MD 21702
⁵ Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital, Boston, MA 02115

CORRESPONDENCE Marie-Claire Gauduin: mcgauduin{at}sfbr.org

We investigated simian immunodeficiency virus (SIV)-specific CD4⁺ T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVnef-infected animals revealed a relatively high frequency of SIV-specific CD4⁺ T cells representing 4–10% of all CD4⁺ T lymphocytes directed against multiple SIV proteins. Gag-specific CD4⁺ T cells in wild-type SIV-infected animals were 5–10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4⁺ cells from SIVnef animals were predominantly CD27^–CD28^–CD45RA^lowCCR7^–CCR5^–, consistent with an effector–memory subset, and included a fully differentiated CD45RA⁺CCR7^– subpopulation. In contrast, SIV-specific CD4⁺ T cells from SIV-infected animals were mostly CD27⁺CD28⁺CD45RA^–CCR7⁺CCR5⁺, consistent with an early central memory phenotype. The CD45RA⁺CCR7^–CD4⁺ subset from SIVnef animals was highly enriched for effector CD4⁺ T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4⁺ T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector–memory T cell population. The ability of SIVnef to induce a high frequency virus-specific CD4⁺ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains.

M.-C. Gauduin's present address is Dept. of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78245.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Reynolds, M. R., Weiler, A. M., Weisgrau, K. L., Piaskowski, S. M., Furlott, J. R., Weinfurter, J. T., Kaizu, M., Soma, T., Leon, E. J., MacNair, C., Leaman, D. P., Zwick, M. B., Gostick, E., Musani, S. K., Price, D. A., Friedrich, T. C., Rakasz, E. G., Wilson, N. A., McDermott, A. B., Boyle, R., Allison, D. B., Burton, D. R., Koff, W. C., Watkins, D. I. (2008). Macaques vaccinated with live-attenuated SIV control replication of heterologous virus. J. Exp. Med. 0: jem.20081524-14 [Abstract] [Full Text]  
• Giraldo-Vela, J. P., Rudersdorf, R., Chung, C., Qi, Y., Wallace, L. T., Bimber, B., Borchardt, G. J., Fisk, D. L., Glidden, C. E., Loffredo, J. T., Piaskowski, S. M., Furlott, J. R., Morales-Martinez, J. P., Wilson, N. A., Rehrauer, W. M., Lifson, J. D., Carrington, M., Watkins, D. I. (2008). The Major Histocompatibility Complex Class II Alleles Mamu-DRB1*1003 and -DRB1*0306 Are Enriched in a Cohort of Simian Immunodeficiency Virus-Infected Rhesus Macaque Elite Controllers. J. Virol. 82: 859-870 [Abstract] [Full Text]  
• Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., Vaslin, B. (2007). Dynamics of T-Cell Responses and Memory T Cells during Primary Simian Immunodeficiency Virus Infection in Cynomolgus Macaques. J. Virol. 81: 13456-13468 [Abstract] [Full Text]  
• Potter, S. J., Lacabaratz, C., Lambotte, O., Perez-Patrigeon, S., Vingert, B., Sinet, M., Colle, J.-H., Urrutia, A., Scott-Algara, D., Boufassa, F., Delfraissy, J.-F., Theze, J., Venet, A., Chakrabarti, L. A. (2007). Preserved Central Memory and Activated Effector Memory CD4+ T-Cell Subsets in Human Immunodeficiency Virus Controllers: an ANRS EP36 Study. J. Virol. 81: 13904-13915 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS