The Journal of Experimental Medicine
CSHL Meetings & Courses
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 7 November 2005. doi:10.1084/jem.20050953
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 202, Number 9, 1279-1288
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Crowe, N. Y.
Right arrow Articles by Smyth, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crowe, N. Y.
Right arrow Articles by Smyth, M. J.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ARTICLE

 Differential antitumor immunity mediated by NKT cell subsets in vivo

Nadine Y. Crowe1, Jonathan M. Coquet1, Stuart P. Berzins1, Konstantinos Kyparissoudis1, Rachael Keating1, Daniel G. Pellicci1, Yoshihiro Hayakawa2, Dale I. Godfrey1, and Mark J. Smyth2

1 Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia
2 Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3002, Australia

CORRESPONDENCE Dale I. Godfrey: godfrey{at}unimelb.edu.au

We showed previously that NKT cell–deficient TCR J{alpha}18–/– mice are more susceptible to methylcholanthrene (MCA)-induced sarcomas, and that normal tumor surveillance can be restored by adoptive transfer of WT liver-derived NKT cells. Liver-derived NKT cells were used in these studies because of their relative abundance in this organ, and it was assumed that they were representative of NKT cells from other sites. We compared NKT cells from liver, thymus, and spleen for their ability to mediate rejection of the sarcoma cell line (MCA-1) in vivo, and found that this was a specialized function of liver-derived NKT cells. Furthermore, when CD4+ and CD4– liver-derived NKT cells were administered separately, MCA-1 rejection was mediated primarily by the CD4– fraction. Very similar results were achieved using the B16F10 melanoma metastasis model, which requires NKT cell stimulation with {alpha}-galactosylceramide. The impaired ability of thymus-derived NKT cells was due, in part, to their production of IL-4, because tumor immunity was clearly enhanced after transfer of IL-4–deficient thymus-derived NKT cells. This is the first study to demonstrate the existence of functionally distinct NKT cell subsets in vivo and may shed light on the long-appreciated paradox that NKT cells function as immunosuppressive cells in some disease models, whereas they promote cell-mediated immunity in others.

Abbreviations used: {alpha}-GalCer, {alpha}-galactosylceramide; CFSE, carboxyfluorescein diacetate succinimidyl ester; MCA, methylcholanthrene, NMS, normal mouse serum.

D.I. Godfrey and M.J. Smyth contributed equally to this work.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

NKT cells get specialized
Heather L. Van Epps
J. Exp. Med. 2005 202: 1156. [Full Text]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS