The Journal of Experimental Medicine
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 18 October 2004. doi:10.1084/jem.20041061
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 200, Number 8, 979-990
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bergman, M. P.
Right arrow Articles by Appelmelk, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bergman, M. P.
Right arrow Articles by Appelmelk, B. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Helicobacter pylori Modulates the T Helper Cell 1/T Helper Cell 2 Balance through Phase-variable Interaction between Lipopolysaccharide and DC-SIGN

Mathijs P. Bergman1,4, Anneke Engering2, Hermelijn H. Smits5, Sandra J. van Vliet2, Ad A. van Bodegraven3, Hans-Peter Wirth6, Martien L. Kapsenberg5, Christina M.J.E. Vandenbroucke-Grauls1, Yvette van Kooyk2, and Ben J. Appelmelk1

1 Department of Medical Microbiology and Infection Control, Vrije Universiteit Medical Center, 1081 BT Amsterdam, Netherlands
2 Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, 1081 BT Amsterdam, Netherlands
3 Department of Gastroenterology, Vrije Universiteit Medical Center, 1081 BT Amsterdam, Netherlands
4 Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, 3015 GD Rotterdam, Netherlands
5 Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands
6 Division of Gastroenterology, GastroZentrum Kreuzlingen, 8280 Kreuzlingen, Switzerland

Address correspondence to Ben J. Appelmelk, Dept. of Medical Microbiology and Infection Control, Vrije Universiteit Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, Netherlands. Phone: 31-20-444-8297; Fax: 31-20-444-8318; email: bj.appelmelk{at}vumc.nl

The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le– variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.

Key Words: Helicobacter pylori • phase variation • DC-SIGN • dendritic cells • Th1/Th2 cell response

M.P. Bergman and A. Engering contributed equally to this work.

Abbreviations used in this paper: AFLP, amplified fragment length polymorphism; Le, Lewis; MOI, multiplicity of infection; MR, mannose receptor; TLR, Toll-like receptor; T reg cell, regulatory T cell.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS