The Journal of Experimental Medicine
Cytokines Montreal 2008
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 15 November 2004. doi:10.1084/jem.20041115
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 200, Number 10, 1337-1346
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, G.
Right arrow Articles by Wahl, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, G.
Right arrow Articles by Wahl, S. M.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection

Ge Ma1, Teresa Greenwell-Wild1, Kejian Lei1, Wenwen Jin1, Jennifer Swisher1, Neil Hardegen1, Carl T. Wild2, and Sharon M. Wahl1

1 Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research (NIDCR), National Institutes of Health (NIH), Bethesda, MD 20892
2 Panacos Pharmaceuticals, Inc., Gaithersburg, MD 20877

Address correspondence to Sharon M. Wahl, Oral Infection and Immunity Branch, NIDCR, NIH, 30 Convent Dr., MSC4352, Building 30, Room 320, Bethesda, MD 20892. Phone: (301) 496-4178; Fax: (301) 402-1064; email: smwahl{at}dir.nidcr.nih.gov

The distribution of secretory leukocyte protease inhibitor (SLPI) at entry portals indicates its involvement in defending the host from pathogens, consistent with the ability of SLPI to inhibit human immunodeficiency virus (HIV)-1 infection by an unknown mechanism. We now demonstrate that SLPI binds to the membrane of human macrophages through the phospholipid-binding protein, annexin II. Based on the recent identification of human cell membrane phosphatidylserine (PS) in the outer coat of HIV-1, we define a novel role for annexin II, a PS-binding moiety, as a cellular cofactor supporting macrophage HIV-1 infection. Moreover, this HIV-1 PS interaction with annexin II can be disrupted by SLPI or other annexin II–specific inhibitors. The PS–annexin II connection may represent a new target to prevent HIV-1 infection.

Key Words: retrovirus • monocytes • AIDS • fusion • phosphatidylserine

G. Ma and T. Greenwell-Wild contributed equally to this work.

Abbreviations used in this paper: LC-MS/MS, nano-capillary HPLC ion trap mass spectrometry; PS, phosphatidylserine; rhSLPI, recombinant human secretory leukocyte protease inhibitor; RNAi, RNA-mediated interference; siRNA, small interfering RNA; SLPI, secretory leukocyte protease inhibitor.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS