The Journal of Experimental Medicine
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 29 December 2003 doi:10.1084/jem.20031582
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 199, Number 1, 131-136
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holtappels, R.
Right arrow Articles by Reddehase, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holtappels, R.
Right arrow Articles by Reddehase, M. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Brief Definitive Report

 Cytomegalovirus Misleads Its Host by Priming of CD8 T Cells Specific for an Epitope Not Presented in Infected Tissues

Rafaela Holtappels1, Jürgen Podlech1, Marcus-Folker Pahl-Seibert1, Markus Jülch2, Doris Thomas1, Christian O. Simon1, Markus Wagner3 and Matthias J. Reddehase1

1 Institute for Virology, Johannes Gutenberg-University, 55101 Mainz, Germany
2 Tumor Vaccination Centre, Johannes Gutenberg-University, 55101 Mainz, Germany
3 Max von Pettenkofer Institute, Department for Virology, Gene Center of the Ludwig Maximilians-University, 81377 Munich, Germany

Address correspondence to Matthias J. Reddehase, Institute for Virology, Johannes Gutenberg-University, Hochhaus am Augustusplatz, 55101 Mainz, Germany. Phone: 49-6131-3933650; Fax: 49-6131-3935604; email: Matthias.Reddehase{at}uni-mainz.de

Cytomegaloviruses (CMVs) code for several proteins that inhibit the presentation of antigenic peptides to CD8 T cells. Although the molecular mechanisms of CMV interference with the major histocompatibility complex class I pathway are long understood, surprisingly little evidence exists to support a role in vivo. Here we document the first example of the presentation of an antigenic peptide being blocked by a CMV immune evasion protein in organs relevant to CMV disease. Although this Db-restricted peptide, which is derived from the antiapoptotic protein M45 of murine CMV (mCMV), is classified as an immunodominant peptide based on response magnitude and long-term memory, adoptive transfer of M45 epitope-specific CD8 T cells did not protect against infection with wild-type mCMV. Notably, the same cells protected C57BL/6 mice infected with an mCMV mutant in which immune evasion protein m152/gp40 is deleted. These data indicate that direct presentation or cross-presentation of an antigenic peptide by professional antigen-presenting cells can efficiently prime CD8 T cells that fail in protection against CMV organ disease because m152/gp40 prevents presentation of this peptide in pathogenetically relevant tissue cells.

Key Words: immunodominance • immune evasion • immune control • antigen presentation • cross-priming

M. Wagner's present address is Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS