The Journal of Experimental Medicine
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Published 2 September 2003. doi:10.1084/jem.20030167
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© Rockefeller University Press, 0022-1007/2003/9/715 $5.00
The Journal of Experimental Medicine, Volume 198, Number 5, 715-724

The Strategy of T Cell Antigen-presenting Cell Encounter in Antigen-draining Lymph Nodes Revealed by Imaging of Initial T Cell Activation

Marc Bajénoff1, Samuel Granjeaud2 and Sylvie Guerder1

1 Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale (INSERM)/Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée, 13288 Marseille, France
2 IFR57, TAGC, INSERM-ERM206, 13288 Marseille, France

Address correspondence to Sylvie Guerder, Centre d'Immunologie de Marseille-Luminy, INSERM/Centre National de la Recherche Scientifique, Université de la Méditerranée, Parc Scientifique de Luminy, Case 906, 13288 Marseille Cedex 09, France. Phone: 33-4-91-26-94-07; Fax: 33-4-91-26-94-30; email: guerder{at}ciml.univ-mrs.fr

The development of an immune response critically relies on the encounter of rare antigen (Ag)-specific T cells with dendritic cells (DCs) presenting the relevant Ag. How two rare cells find each other in the midst of irrelevant other cells in lymph nodes (LNs) is unknown. Here we show that initial T cell activation clusters are generated near high endothelial venules (HEVs) in the outer paracortex of draining LNs by retention of Ag-specific T cells as they exit from HEVs. We further show that tissue-derived DCs preferentially home in the vicinity of HEVs, thus defining the site of cluster generation. At this location DCs efficiently scan all incoming T cells and selectively retain those specific for the major histocompatibility complex–peptide complexes the DCs present. Such strategic positioning of DCs on the entry route of T cells into the paracortex may foster T cell–DC encounter and thus optimize initial T cell activation in vivo.

Key Words: T cell activation • dendritic cells • imaging • in vivo


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