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Nadda Brdiková¹, Tomá Brdika¹, Pavla Angelisová¹, Ondrej Horváth¹, Jií pika¹, Ivan Hilgert¹, Jan Paes¹, Luca Simeoni², Stefanie Kliche², Camilla Merten², Burkhart Schraven² and Václav Hoejí¹

¹ Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
² Institute for Immunology, Otto-von-Guericke-University, 39120 Magdeburg, Germany

Address correspondence to Václav Hoejí, Institute of Molecular Genetics, AS CR, Vídenská 1083, 142 20 Praha 4, Czech Republic. Phone: 420-2-41729908; Fax: 420-2-44472282; email: horejsi{at}biomed.cas.cz; or Burkhart Schraven, Institute for Immunology, Otto-von-Guericke-University, Leipziger Strasse 44, 39120 Magdeburg, Germany. Phone: 0391-67-15800; Fax: 0391-67-15852; email: burkhart.schraven{at}medizin.uni-magdeburg.de

Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca²⁺ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.

Key Words: membrane microdomains • Lck • Csk • signal transduction • phosphorylation

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