The Journal of Experimental Medicine
ThymUS '08
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Published 6 May 2002. doi:10.1084/jem.20011284
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© Rockefeller University Press, 0022-1007/2002/5/1145/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 9, May 6, 2002 1145-1154
Hematopoietic Stem Cells Are Uniquely Selective in Their Migratory Response to Chemokines

Douglas E. Wright1, Edward P. Bowman2,3,4,5, Amy J. Wagers1, Eugene C. Butcher2,3,4 and Irving L. Weissman1

1 Departments of Pathology and Developmental Biology, Department of Pathology
2 Laboratory of Immunology and Vascular Biology, Department of Pathology
3 Digestive Disease Center, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305
4 Center for Molecular Biology and Medicine, Veterans Affairs, Palo Alto Health Care System, Palo Alto, CA 94305
5 Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304

Address correspondence to Douglas E. Wright, Department of Pathology (5324), Beckman B-261, 279 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305. Phone: 650-725-5808; Fax: 650-498-6255; E-mail: doug.wright{at}stanford.edu

Although hematopoietic stem cell (HSC) migration into and out of sites of active hematopoiesis is poorly understood, it is a critical process that underlies modern clinical stem cell transplantation and may be important for normal hematopoietic homeostasis. Given the established roles of chemotactic cytokine (chemokine)-directed migration of other leukocyte subsets, the migration of murine HSC to a large panel of CC and CXC chemokines was investigated. HSC migrated only in response to stromal derived factor-1{alpha}, the ligand for the CXC chemokine receptor 4 (CXCR4). CXCR4 expression by HSC was confirmed by reverse transcription polymerase chain reaction analysis. Surprisingly, HSC also expressed mRNA for CCR3 and CCR9, although they failed to migrate to the ligands for these receptors. The sharply restricted chemotactic responsiveness of HSC is unique among leukocytes and may be necessary for the specific homing of circulating HSC to bone marrow, as well as for the maintenance of HSC in hematopoietic microenvironments.

Key Words: chemokines • chemokine receptors • chemotaxis • mobilization • bone marrow


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