Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mori, Y. Articles by Kurosaki, T. Search for Related Content PubMed PubMed Citation Articles by Mori, Y. Articles by Kurosaki, T. Social Bookmarking                      What's this?

Transient Receptor Potential 1 Regulates Capacitative Ca²⁺ Entry and Ca²⁺ Release from Endoplasmic Reticulum in B Lymphocytes

¹ Center for Integrative Bioscience and Department of Information Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
² Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, CREST, Japan Science and Technology Corporation, Tokyo 113-0033, Japan
³ Laboratory of Cell and Molecular Signaling, Center for Biomedical Research at The Queen's Medical Center, and John A. Burns School of Medicine at the University of Hawaii, Honolulu, HI 96813
⁴ Department of Molecular Genetics, Institute for Liver Research, Kansai Medical University, and Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Moriguchi 570-8506, Japan

Address correspondence to Yasuo Mori, Center for Integrative Bioscience, National Institute for Physiological Sciences, Myodaiji-cho, Okazaki 444-8585, Japan. Phone: 81-564-55-7722; Fax: 81-564-55-7721; E-mail: moriy{at}nips.ac.jp or Tomohiro Kurosaki, Dept. of Molecular Genetics, Institute for Liver Research, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi 570-8506, Japan. Phone: 81-6-6993-9445; Fax: 81-6-6994-6099; E-mail: kurosaki{at}mesh.ne.jp

Capacitative Ca²⁺ entry (CCE) activated by release/depletion of Ca²⁺ from internal stores represents a major Ca²⁺ influx mechanism in lymphocytes and other nonexcitable cells. Despite the importance of CCE in antigen-mediated lymphocyte activation, molecular components constituting this mechanism remain elusive. Here we demonstrate that genetic disruption of transient receptor potential (TRP)1 significantly attenuates both Ca²⁺ release-activated Ca²⁺ currents and inositol 1,4,5-trisphosphate (IP₃)-mediated Ca²⁺ release from endoplasmic reticulum (ER) in DT40 B cells. As a consequence, B cell antigen receptor–mediated Ca²⁺ oscillations and NF-AT activation are reduced in TRP1-deficient cells. Thus, our results suggest that CCE channels, whose formation involves TRP1 as an important component, modulate IP₃ receptor function, thereby enhancing functional coupling between the ER and plasma membrane in transduction of intracellular Ca²⁺ signaling in B lymphocytes.

Key Words: B cell receptor • capacitative Ca²⁺ entry • store-operated Ca²⁺ channel • Ca²⁺ release • TRP

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Liu, X., Cheng, K. T., Bandyopadhyay, B. C., Pani, B., Dietrich, A., Paria, B. C., Swaim, W. D., Beech, D., Yildrim, E., Singh, B. B., Birnbaumer, L., Ambudkar, I. S. (2007). Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1( / ) mice. Proc. Natl. Acad. Sci. USA 104: 17542-17547 [Abstract] [Full Text]  
• Putney, J. W. Jr (2007). New molecular players in capacitative Ca2+ entry. J. Cell Sci. 120: 1959-1965 [Abstract] [Full Text]  
• Chung, S. C., Limnander, A., Kurosaki, T., Weiss, A., Korenbrot, J. I. (2007). Coupling Ca2+ store release to Icrac channel activation in B lymphocytes requires the activity of Lyn and Syk kinases. J. Cell Biol. 177: 317-328 [Abstract] [Full Text]  
• Ong, H. L., Cheng, K. T., Liu, X., Bandyopadhyay, B. C., Paria, B. C., Soboloff, J., Pani, B., Gwack, Y., Srikanth, S., Singh, B. B., Gill, D., Ambudkar, I. S. (2007). Dynamic Assembly of TRPC1-STIM1-Orai1 Ternary Complex Is Involved in Store-operated Calcium Influx: EVIDENCE FOR SIMILARITIES IN STORE-OPERATED AND CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL COMPONENTS. J. Biol. Chem. 282: 9105-9116 [Abstract] [Full Text]  
• Nilius, B., Owsianik, G., Voets, T., Peters, J. A. (2007). Transient Receptor Potential Cation Channels in Disease. Physiol. Rev. 87: 165-217 [Abstract] [Full Text]  
• Carroll, H. P., McNaull, B. B.A., Gadina, M. (2006). Immunodeficiency Is A Tough Nut to CRAC: The Importance of Calcium Flux in T Cell Activation.. Mol. Interv. 6: 253-256 [Abstract] [Full Text]  
• Smyth, J. T., Lemonnier, L., Vazquez, G., Bird, G. S., Putney, J. W. Jr. (2006). Dissociation of Regulated Trafficking of TRPC3 Channels to the Plasma Membrane from Their Activation by Phospholipase C. J. Biol. Chem. 281: 11712-11720 [Abstract] [Full Text]  
• Zhang, L., Guo, F., Kim, J. Y., Saffen, D. (2006). Muscarinic Acetylcholine Receptors Activate TRPC6 Channels in PC12D Cells via Ca2+ Store-Independent Mechanisms.. J Biochem 139: 459-470 [Abstract] [Full Text]  
• Balla, T (2006). Phosphoinositide-derived messengers in endocrine signaling. J Endocrinol 188: 135-153 [Abstract] [Full Text]  
• Rao, G. K., Kaminski, N. E. (2006). Induction of intracellular calcium elevation by {Delta}9-tetrahydrocannabinol in T cells involves TRPC1 channels. J. Leukoc. Biol. 79: 202-213 [Abstract] [Full Text]  
• Lievremont, J.-P., Numaga, T., Vazquez, G., Lemonnier, L., Hara, Y., Mori, E., Trebak, M., Moss, S. E., Bird, G. S., Mori, Y., Putney, J. W. Jr. (2005). The Role of Canonical Transient Receptor Potential 7 in B-cell Receptor-activated Channels. J. Biol. Chem. 280: 35346-35351 [Abstract] [Full Text]  
• Feske, S., Prakriya, M., Rao, A., Lewis, R. S. (2005). A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients. J. Exp. Med. 202: 651-662 [Abstract] [Full Text]  
• Freichel, M., Vennekens, R., Olausson, J., Stolz, S., Philipp, S. E, Weissgerber, P., Flockerzi, V. (2005). Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies. J. Physiol. 567: 59-66 [Abstract] [Full Text]  
• Roos, J., DiGregorio, P. J., Yeromin, A. V., Ohlsen, K., Lioudyno, M., Zhang, S., Safrina, O., Kozak, J. A., Wagner, S. L., Cahalan, M. D., Velicelebi, G., Stauderman, K. A. (2005). STIM1, an essential and conserved component of store-operated Ca2+ channel function. J. Cell Biol. 169: 435-445 [Abstract] [Full Text]  
• Parekh, A. B., Putney, J. W. Jr. (2005). Store-Operated Calcium Channels. Physiol. Rev. 85: 757-810 [Abstract] [Full Text]  
• Liu, Q.-H., Liu, X., Wen, Z., Hondowicz, B., King, L., Monroe, J., Freedman, B. D. (2005). Distinct Calcium Channels Regulate Responses of Primary B Lymphocytes to B Cell Receptor Engagement and Mechanical Stimuli. J. Immunol. 174: 68-79 [Abstract] [Full Text]  
• Yeromin, A. V., Roos, J., Stauderman, K. A., Cahalan, M. D. (2004). A Store-operated Calcium Channel in Drosophila S2 Cells. J. Gen. Physiol. 123: 167-182 [Abstract] [Full Text]  
• Itagaki, K., Kannan, K. B., Singh, B. B., Hauser, C. J. (2004). Cytoskeletal Reorganization Internalizes Multiple Transient Receptor Potential Channels and Blocks Calcium Entry into Human Neutrophils. J. Immunol. 172: 601-607 [Abstract] [Full Text]  
• Ma, R., Rundle, D., Jacks, J., Koch, M., Downs, T., Tsiokas, L. (2003). Inhibitor of Myogenic Family, a Novel Suppressor of Store-operated Currents through an Interaction with TRPC1. J. Biol. Chem. 278: 52763-52772 [Abstract] [Full Text]  
• Huang, T. T., Zong, Y., Dalwadi, H., Chung, C., Miceli, M. C., Spicher, K., Birnbaumer, L., Braun, J., Aranda, R. (2003). TCR-mediated hyper-responsiveness of autoimmune G{alpha}i2-/- mice is an intrinsic naive CD4+ T cell disorder selective for the G{alpha}i2 subunit. Int Immunol 15: 1359-1367 [Abstract] [Full Text]  
• Liu, W., Xu, S., Woda, C., Kim, P., Weinbaum, S., Satlin, L. M. (2003). Effect of flow and stretch on the [Ca2+]i response of principal and intercalated cells in cortical collecting duct. Am. J. Physiol. Renal Physiol. 285: F998-F1012 [Abstract] [Full Text]  
• Li, H., Ayer, L. M., Lytton, J., Deans, J. P. (2003). Store-operated Cation Entry Mediated by CD20 in Membrane Rafts. J. Biol. Chem. 278: 42427-42434 [Abstract] [Full Text]  
• Philipp, S., Strauss, B., Hirnet, D., Wissenbach, U., Mery, L., Flockerzi, V., Hoth, M. (2003). TRPC3 Mediates T-cell Receptor-dependent Calcium Entry in Human T-lymphocytes. J. Biol. Chem. 278: 26629-26638 [Abstract] [Full Text]  
• Brazer, S.-c. W., Singh, B. B., Liu, X., Swaim, W., Ambudkar, I. S. (2003). Caveolin-1 Contributes to Assembly of Store-operated Ca2+ Influx Channels by Regulating Plasma Membrane Localization of TRPC1. J. Biol. Chem. 278: 27208-27215 [Abstract] [Full Text]  
• Gusev, K., Glouchankova, L., Zubov, A., Kaznacheyeva, E., Wang, Z., Bezprozvanny, I., Mozhayeva, G. N. (2003). The Store-operated Calcium Entry Pathways in Human Carcinoma A431 Cells: Functional Properties and Activation Mechanisms. J. Gen. Physiol. 122: 81-94 [Abstract] [Full Text]  
• Liu, X., Singh, B. B., Ambudkar, I. S. (2003). TRPC1 Is Required for Functional Store-operated Ca2+ Channels. ROLE OF ACIDIC AMINO ACID RESIDUES IN THE S5-S6 REGION. J. Biol. Chem. 278: 11337-11343 [Abstract] [Full Text]  
• Sage, S. O., Brownlow, S. L., Rosado, J. A., Authi, K. S., Hassock, S., Zhu, M. X., Flockerzi, V., Trost, C. (2002). TRP channels and calcium entry in human platelets. Blood 100: 4245-4246 [Full Text]  
• Rosado, J. A., Brownlow, S. L., Sage, S. O. (2002). Endogenously Expressed Trp1 Is Involved in Store-mediated Ca2+ Entry by Conformational Coupling in Human Platelets. J. Biol. Chem. 277: 42157-42163 [Abstract] [Full Text]  
• Feng, P., Park, J., Lee, B.-S., Lee, S.-H., Bram, R. J., Jung, J. U. (2002). Kaposi's Sarcoma-Associated Herpesvirus Mitochondrial K7 Protein Targets a Cellular Calcium-Modulating Cyclophilin Ligand To Modulate Intracellular Calcium Concentration and Inhibit Apoptosis. J. Virol. 76: 11491-11504 [Abstract] [Full Text]  
• Hassock, S. R., Zhu, M. X., Trost, C., Flockerzi, V., Authi, K. S. (2002). Expression and role of TRPC proteins in human platelets: evidence that TRPC6 forms the store-independent calcium entry channel. Blood 100: 2801-2811 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS