The Journal of Experimental Medicine
Tools for dendritic cell research
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 20 May 2002. doi:10.1084/jem.20010753
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sgarbanti, M.
Right arrow Articles by Battistini, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sgarbanti, M.
Right arrow Articles by Battistini, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© Rockefeller University Press, 0022-1007/2002/5/1359/ $5.00
The Journal of Experimental Medicine, Volume 195, Number 10, May 20, 2002 1359-1370
Modulation of Human Immunodeficiency Virus 1 Replication by Interferon Regulatory Factors

Marco Sgarbanti1, Alessandra Borsetti1, Nicola Moscufo2, Maria C. Bellocchi1, Barbara Ridolfi1, Filomena Nappi1, Giulia Marsili1, Giovanna Marziali1, Eliana M. Coccia3, Barbara Ensoli1 and Angela Battistini1

1 Laboratory of Virology, Istituto Superiore di Sanità, 00161 Rome, Italy
2 Laboratory of Cellular Biology, Istituto Superiore di Sanità, 00161 Rome, Italy
3 Laboratory of Immunology, Istituto Superiore di Sanità, 00161 Rome, Italy

Address correspondence to A. Battistini, Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-06-49903266; Fax: 39-06-49902082; E-mail: battist{at}iss.it or to Dr. B. Ensoli, Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-06-49903208; Fax: 39-06-49903002; E-mail: ensoli{at}iss.it

Transcription of the human immunodeficiency virus (HIV)-1 is controlled by the cooperation of virally encoded and host regulatory proteins. The Tat protein is essential for viral replication, however, expression of Tat after virus entry requires HIV-1 promoter activation. A sequence in the 5' HIV-1 LTR, containing a binding site for transcription factors of the interferon regulatory factors (IRF) family has been suggested to be critical for HIV-1 transcription and replication. Here we show that IRF-1 activates HIV-1 LTR transcription in a dose-dependent fashion and in the absence of Tat. This has biological significance since IRF-1 is produced early upon virus entry, both in cell lines and in primary CD4+ T cells, and before expression of Tat. IRF-1 also cooperates with Tat in amplifying virus gene transcription and replication. This cooperation depends upon a physical interaction that is blocked by overexpression of IRF-8, the natural repressor of IRF-1, and, in turn is released by overexpression of IRF-1. These data suggest a key role of IRF-1 in the early phase of viral replication and/or during viral reactivation from latency, when viral transactivators are absent or present at very low levels, and suggest that the interplay between IRF-1 and IRF-8 may play a key role in virus latency.

Key Words: virus replication • Tat • transcription factors • gene expression • T lymphocytes


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS