Homeostasis of Peripheral B Cells in the Absence of B Cell Influx from the Bone Marrow

doi:10.1084/jem.194.8.1151

Published 15 October 2001. doi:10.1084/jem.194.8.1151

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© The Rockefeller University Press, 0022-1007/2001/10/1151/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 8, October 15, 2001 1151-1164

Original Article

Homeostasis of Peripheral B Cells in the Absence of B Cell Influx from the Bone Marrow

Zhenyue Hao^a and Klaus Rajewsky^a
^a Department of Immunology, Institute for Genetics, University of Cologne, D-50931 Cologne, Germany

Correspondence to: Klaus Rajewsky, Department of Immunology, Institute for Genetics, University of Cologne, Weyertal 121, D-50931 Cologne, Germany. Tel:49-221-4702467 Fax:49-221-4705185 E-mail:klaus.rajewsky{at}mac.genetik.uni-koeln.de.

To study homeostasis of peripheral B lymphocytes in the absenceof B cell influx from the bone marrow, we generated a mousemutant in which the recombination-activating gene (RAG)-2 canbe inducibly deleted. When RAG-2 was deleted at the age of 8–10wk, splenic naive follicular B cells were gradually lost overa year of observation, with a half-life of $~$ 4.5 mo. By contrast,the pool of marginal zone B cells in the spleen and of B-1 cellsin the peritoneal cavity were kept at normal level. In lymphnodes, $~$ 90% of the B cells were lost within 4 mo, and B cellnumbers remained constant thereafter. Mice in which RAG-2 wasdeleted at birth maintained a small population of activatedB cells with an increased proportion of marginal zone B cells.Additionally, an increase of the pool of IgM secreting cellsand B-1a cells was observed.

Key Words: RAG-2, mouse, marginal zone B cells, follicular B cells, half-life

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