The Journal of Experimental Medicine
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 17 December 2001. doi:10.1084/jem.194.12.1813
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lechmann, M.
Right arrow Articles by Steinkasserer, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lechmann, M.
Right arrow Articles by Steinkasserer, A.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© Rockefeller University Press, 0022-1007/2001/12/1813/ $5.00
The Journal of Experimental Medicine, Volume 194, Number 12, December 17, 2001 1813-1821

Original Article

 The Extracellular Domain of CD83 Inhibits Dendritic Cell–mediated T Cell Stimulation and Binds to a Ligand on Dendritic Cells

Matthias Lechmann1, Daniëlle J.E.B. Krooshoop2, Diana Dudziak3, Elisabeth Kremmer3,4, Christine Kuhnt1, Carl G. Figdor2, Gerold Schuler1 and Alexander Steinkasserer1

1 Department of Dermatology, University of Erlangen-Nuremberg, D-91052 Erlangen, Germany
2 Department of Tumor Immunology, NCMLS/187 Til, University Medical Center, 6500HB Nijmegen, Netherlands
3 Institute of Clinical Molecular Biology and Tumor Genetik, GSF-Forschungszentrum fur Umwelt und Gesundheit, GmbH, D-81377 Munich, Germany
4 Institute of Molecular Immunology, GSF-Forschungszentrum fur Umwelt und Gesundheit, GmbH, D-81377 Munich, Germany

Address correspondence to Alexander Steinkasserer, Dept. of Dermatology, Hartmannstrasse 14, D-91052 Erlangen, Germany. Phone: 49-9131-853-6725; Fax: 49-9131-853-5799 or 49-9131-85-6175; E-mail: steinkasserer{at}derma.med.uni-erlangen.de

CD83 is an immunoglobulin (Ig) superfamily member that is upregulated during the maturation of dendritic cells (DCs). It has been widely used as a marker for mature DCs, but its function is still unknown. To approach its potential functional role, we have expressed the extracellular Ig domain of human CD83 (hCD83ext) as a soluble protein. Using this tool we could show that immature as well as mature DCs bind to CD83. Since CD83 binds a ligand also expressed on immature DCs, which do not express CD83, indicates that binding is not a homophilic interaction. In addition we demonstrate that hCD83ext interferes with DC maturation downmodulating the expression of CD80 and CD83, while no phenotypical effects were observed on T cells. Finally, we show that hCD83ext inhibits DC-dependent allogeneic and peptide-specific T cell proliferation in a concentration dependent manner in vitro. This is the first report regarding functional aspects of CD83 and the binding of CD83 to DCs.

Key Words: CD83 • dendritic cells • MLR • T cell inhibition • recombinant expression


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS