Identification of a Chlamydial Protease-like Activity Factor Responsible for the Degradation of Host Transcription Factors

doi:10.1084/jem.193.8.935

Published online 16 April 2001.

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Zhong, G.
	Articles by Huang, Y.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Zhong, G.
	Articles by Huang, Y.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0022-1007/2001/4/935/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 8, April 16, 2001 935-942

Original Article

Identification of a Chlamydial Protease–like Activity Factor Responsible for the Degradation of Host Transcription Factors

Guangming Zhong^a, Peiyi Fan^a, Hezhao Ji^b, Feng Dong^a, and Yanqing Huang^a
^a Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229
^b Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3E OW3, Canada

Correspondence to: Guangming Zhong, Department of Microbiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229. Tel:210-567-1169 Fax:210-567-0293 E-mail:zhongg{at}uthscsa.edu.

Microbial pathogens have been selected for the capacity to evadeor manipulate host responses in order to survive after infection.Chlamydia, an obligate intracellular pathogen and the causativeagent for many human diseases, can escape T lymphocyte immunerecognition by degrading host transcription factors requiredfor major histocompatibility complex (MHC) antigen expression.We have now identified a chlamydial protease– or proteasome–likeactivity factor (CPAF) that is secreted into the host cell cytosoland that is both necessary and sufficient for the degradationof host transcription factors RFX5 and upstream stimulationfactor 1 (USF-1). The CPAF gene is highly conserved among chlamydialstrains, but has no significant overall homology with otherknown genes. Thus, CPAF represents a unique secreted proteinproduced by an obligate intracellular bacterial pathogen tointerfere with effective host adaptive immunity.

Key Words: chlamydial enzyme, degradation, transcription factors, antigenpresentation, immune evasion

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Jorgensen, I., Valdivia, R. H. (2008). Pmp-Like Proteins Pls1 and Pls2 Are Secreted into the Lumen of the Chlamydia trachomatis Inclusion. Infect. Immun. 76: 3940-3950 [Abstract] [Full Text]
Li, Z., Chen, D., Zhong, Y., Wang, S., Zhong, G. (2008). The Chlamydial Plasmid-Encoded Protein pgp3 Is Secreted into the Cytosol of Chlamydia-Infected Cells. Infect. Immun. 76: 3415-3428 [Abstract] [Full Text]
Paschen, S. A., Christian, J. G., Vier, J., Schmidt, F., Walch, A., Ojcius, D. M., Hacker, G. (2008). Cytopathicity of Chlamydia is largely reproduced by expression of a single chlamydial protease. J. Cell Biol. 182: 117-127 [Abstract] [Full Text]
Li, Z., Chen, C., Chen, D., Wu, Y., Zhong, Y., Zhong, G. (2008). Characterization of Fifty Putative Inclusion Membrane Proteins Encoded in the Chlamydia trachomatis Genome. Infect. Immun. 76: 2746-2757 [Abstract] [Full Text]
Johnson, D. L., Stone, C. B., Mahony, J. B. (2008). Interactions between CdsD, CdsQ, and CdsL, Three Putative Chlamydophila pneumoniae Type III Secretion Proteins. J. Bacteriol. 190: 2972-2980 [Abstract] [Full Text]
Paland, N., Bohme, L., Gurumurthy, R. K., Maurer, A., Szczepek, A. J., Rudel, T. (2008). Reduced Display of Tumor Necrosis Factor Receptor I at the Host Cell Surface Supports Infection with Chlamydia trachomatis. J. Biol. Chem. 283: 6438-6448 [Abstract] [Full Text]
Cheng, W., Shivshankar, P., Zhong, Y., Chen, D., Li, Z., Zhong, G. (2008). Intracellular Interleukin-1{alpha} Mediates Interleukin-8 Production Induced by Chlamydia trachomatis Infection via a Mechanism Independent of Type I Interleukin-1 Receptor. Infect. Immun. 76: 942-951 [Abstract] [Full Text]
Li, W., Murthy, A. K., Guentzel, M. N., Seshu, J., Forsthuber, T. G., Zhong, G., Arulanandam, B. P. (2008). Antigen-Specific CD4+ T Cells Produce Sufficient IFN-{gamma} to Mediate Robust Protective Immunity against Genital Chlamydia muridarum Infection. J. Immunol. 180: 3375-3382 [Abstract] [Full Text]
Cheng, W., Shivshankar, P., Li, Z., Chen, L., Yeh, I-T., Zhong, G. (2008). Caspase-1 Contributes to Chlamydia trachomatis-Induced Upper Urogenital Tract Inflammatory Pathologies without Affecting the Course of Infection. Infect. Immun. 76: 515-522 [Abstract] [Full Text]
Cragnolini, J. J., de Castro, J. A. L. (2008). Identification of Endogenously Presented Peptides from Chlamydia trachomatis with High Homology to Human Proteins and to a Natural Self-ligand of HLA-B27. Mol. Cell. Proteomics 7: 170-180 [Abstract] [Full Text]
Li, W., Guentzel, M. N., Seshu, J., Zhong, G., Murthy, A. K., Arulanandam, B. P. (2007). Induction of Cross-Serovar Protection against Genital Chlamydial Infection by a Targeted Multisubunit Vaccination Approach. CVI 14: 1537-1544 [Abstract] [Full Text]
Lad, S. P., Yang, G., Scott, D. A., Wang, G., Nair, P., Mathison, J., Reddy, V. S., Li, E. (2007). Chlamydial CT441 Is a PDZ Domain-Containing Tail-Specific Protease That Interferes with the NF-{kappa}B Pathway of Immune Response. J. Bacteriol. 189: 6619-6625 [Abstract] [Full Text]
Hermann, C. (2007). Review: Variability of host pathogen interaction. Innate Immunity 13: 199-218 [Abstract]
Hybiske, K., Stephens, R. S. (2007). Mechanisms of host cell exit by the intracellular bacterium Chlamydia. Proc. Natl. Acad. Sci. USA 104: 11430-11435 [Abstract] [Full Text]
Wang, G., Burczynski, F., Anderson, J., Zhong, G. (2007). Effect of host fatty acid-binding protein and fatty acid uptake on growth of Chlamydia trachomatis L2. Microbiology 153: 1935-1939 [Abstract] [Full Text]
Kawana, K., Quayle, A. J., Ficarra, M., Ibana, J. A., Shen, L., Kawana, Y., Yang, H., Marrero, L., Yavagal, S., Greene, S. J., Zhang, Y.-X., Pyles, R. B., Blumberg, R. S., Schust, D. J. (2007). CD1d Degradation in Chlamydia trachomatis-infected Epithelial Cells Is the Result of Both Cellular and Chlamydial Proteasomal Activity. J. Biol. Chem. 282: 7368-7375 [Abstract] [Full Text]
Flores, R., Luo, J., Chen, D., Sturgeon, G., Shivshankar, P., Zhong, Y., Zhong, G. (2007). Characterization of the hypothetical protein Cpn1027, a newly identified inclusion membrane protein unique to Chlamydia pneumoniae. Microbiology 153: 777-786 [Abstract] [Full Text]
Lad, S. P., Li, J., da Silva Correia, J., Pan, Q., Gadwal, S., Ulevitch, R. J., Li, E. (2007). Cleavage of p65/RelA of the NF-{kappa}B pathway by Chlamydia. Proc. Natl. Acad. Sci. USA 104: 2933-2938 [Abstract] [Full Text]
Murthy, A. K., Chambers, J. P., Meier, P. A., Zhong, G., Arulanandam, B. P. (2007). Intranasal Vaccination with a Secreted Chlamydial Protein Enhances Resolution of Genital Chlamydia muridarum Infection, Protects against Oviduct Pathology, and Is Highly Dependent upon Endogenous Gamma Interferon Production. Infect. Immun. 75: 666-676 [Abstract] [Full Text]
Luo, J., Jia, T., Flores, R., Chen, D., Zhong, G. (2007). Hypothetical Protein Cpn0308 Is Localized in the Chlamydia pneumoniae Inclusion Membrane. Infect. Immun. 75: 497-503 [Abstract] [Full Text]
Yu, H. H. Y., Kibler, D., Tan, M. (2006). In Silico Prediction and Functional Validation of {sigma}28-Regulated Genes in Chlamydia and Escherichia coli. J. Bacteriol. 188: 8206-8212 [Abstract] [Full Text]
Murthy, A. K., Cong, Y., Murphey, C., Guentzel, M. N., Forsthuber, T. G., Zhong, G., Arulanandam, B. P. (2006). Chlamydial Protease-Like Activity Factor Induces Protective Immunity against Genital Chlamydial Infection in Transgenic Mice That Express the Human HLA-DR4 Allele. Infect. Immun. 74: 6722-6729 [Abstract] [Full Text]
Dong, F., Flores, R., Chen, D., Luo, J., Zhong, Y., Wu, Z., Zhong, G. (2006). Localization of the Hypothetical Protein Cpn0797 in the Cytoplasm of Chlamydia pneumoniae-Infected Host Cells.. Infect. Immun. 74: 6479-6486 [Abstract] [Full Text]
Goldschmidt, P, Rostane, H, Sow, M, Goepogui, A, Batellier, L, Chaumeil, C (2006). Detection by broad-range real-time PCR assay of Chlamydia species infecting human and animals. Br. J. Ophthalmol. 90: 1425-1429 [Abstract] [Full Text]
Pirbhai, M., Dong, F., Zhong, Y., Pan, K. Z., Zhong, G. (2006). The Secreted Protease Factor CPAF Is Responsible for Degrading Pro-apoptotic BH3-only Proteins in Chlamydia trachomatis-infected Cells. J. Biol. Chem. 281: 31495-31501 [Abstract] [Full Text]
Balsara, Z. R., Misaghi, S., Lafave, J. N., Starnbach, M. N. (2006). Chlamydia trachomatis Infection Induces Cleavage of the Mitotic Cyclin B1.. Infect. Immun. 74: 5602-5608 [Abstract] [Full Text]
Muschiol, S., Bailey, L., Gylfe, A., Sundin, C., Hultenby, K., Bergstrom, S., Elofsson, M., Wolf-Watz, H., Normark, S., Henriques-Normark, B. (2006). A small-molecule inhibitor of type III secretion inhibits different stages of the infectious cycle of Chlamydia trachomatis. Proc. Natl. Acad. Sci. USA 103: 14566-14571 [Abstract] [Full Text]
Chen, C., Chen, D., Sharma, J., Cheng, W., Zhong, Y., Liu, K., Jensen, J., Shain, R., Arulanandam, B., Zhong, G. (2006). The Hypothetical Protein CT813 Is Localized in the Chlamydia trachomatis Inclusion Membrane and Is Immunogenic in Women Urogenitally Infected with C. trachomatis.. Infect. Immun. 74: 4826-4840 [Abstract] [Full Text]
Sharma, J., Zhong, Y., Dong, F., Piper, J. M., Wang, G., Zhong, G. (2006). Profiling of Human Antibody Responses to Chlamydia trachomatis Urogenital Tract Infection Using Microplates Arrayed with 156 Chlamydial Fusion Proteins. Infect. Immun. 74: 1490-1499 [Abstract] [Full Text]
Suchland, R. J., Rockey, D. D., Weeks, S. K., Alzhanov, D. T., Stamm, W. E. (2005). Development of Secondary Inclusions in Cells Infected by Chlamydia trachomatis. Infect. Immun. 73: 3954-3962 [Abstract] [Full Text]
Sharma, J., Dong, F., Pirbhai, M., Zhong, G. (2005). Inhibition of Proteolytic Activity of a Chlamydial Proteasome/Protease-Like Activity Factor by Antibodies from Humans Infected with Chlamydia trachomatis. Infect. Immun. 73: 4414-4419 [Abstract] [Full Text]
Dong, F., Pirbhai, M., Xiao, Y., Zhong, Y., Wu, Y., Zhong, G. (2005). Degradation of the Proapoptotic Proteins Bik, Puma, and Bim with Bcl-2 Domain 3 Homology in Chlamydia trachomatis-Infected Cells. Infect. Immun. 73: 1861-1864 [Abstract] [Full Text]
Dong, F., Zhong, Y., Arulanandam, B., Zhong, G. (2005). Production of a Proteolytically Active Protein, Chlamydial Protease/Proteasome-Like Activity Factor, by Five Different Chlamydia Species. Infect. Immun. 73: 1868-1872 [Abstract] [Full Text]
Xiao, Y., Zhong, Y., Su, H., Zhou, Z., Chiao, P., Zhong, G. (2005). NF-{kappa}B Activation Is Not Required for Chlamydia trachomatis Inhibition of Host Epithelial Cell Apoptosis. J. Immunol. 174: 1701-1708 [Abstract] [Full Text]
Collingro, A., Poppert, S., Heinz, E., Schmitz-Esser, S., Essig, A., Schweikert, M., Wagner, M., Horn, M. (2005). Recovery of an environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp.. Microbiology 151: 301-309 [Abstract] [Full Text]
Sharma, J., Bosnic, A. M., Piper, J. M., Zhong, G. (2004). Human Antibody Responses to a Chlamydia-Secreted Protease Factor. Infect. Immun. 72: 7164-7171 [Abstract] [Full Text]
Steele, L. N., Balsara, Z. R., Starnbach, M. N. (2004). Hematopoietic Cells Are Required to Initiate a Chlamydia trachomatis-Specific CD8+ T Cell Response. J. Immunol. 173: 6327-6337 [Abstract] [Full Text]
Fischer, S. F., Vier, J., Kirschnek, S., Klos, A., Hess, S., Ying, S., Hacker, G. (2004). Chlamydia Inhibit Host Cell Apoptosis by Degradation of Proapoptotic BH3-only Proteins. J. Exp. Med. 200: 905-916 [Abstract] [Full Text]
Xiao, Y., Zhong, Y., Greene, W., Dong, F., Zhong, G. (2004). Chlamydia trachomatis Infection Inhibits Both Bax and Bak Activation Induced by Staurosporine. Infect. Immun. 72: 5470-5474 [Abstract] [Full Text]
Dong, F., Su, H., Huang, Y., Zhong, Y., Zhong, G. (2004). Cleavage of Host Keratin 8 by a Chlamydia-Secreted Protease. Infect. Immun. 72: 3863-3868 [Abstract] [Full Text]
Dong, F., Sharma, J., Xiao, Y., Zhong, Y., Zhong, G. (2004). Intramolecular Dimerization Is Required for the Chlamydia-Secreted Protease CPAF To Degrade Host Transcriptional Factors. Infect. Immun. 72: 3869-3875 [Abstract] [Full Text]
Nagarajan, U. M., Long, A. B., Harreman, M. T., Corbett, A. H., Boss, J. M. (2004). A Hierarchy of Nuclear Localization Signals Governs the Import of the Regulatory Factor X Complex Subunits and MHC Class II Expression. J. Immunol. 173: 410-419 [Abstract] [Full Text]
Horn, M., Collingro, A., Schmitz-Esser, S., Beier, C. L., Purkhold, U., Fartmann, B., Brandt, P., Nyakatura, G. J., Droege, M., Frishman, D., Rattei, T., Mewes, H.-W., Wagner, M. (2004). Illuminating the Evolutionary History of Chlamydiae. Science 304: 728-730 [Abstract] [Full Text]
Hogan, R. J., Mathews, S. A., Mukhopadhyay, S., Summersgill, J. T., Timms, P. (2004). Chlamydial Persistence: beyond the Biphasic Paradigm. Infect. Immun. 72: 1843-1855 [Full Text]
Su, H., McClarty, G., Dong, F., Hatch, G. M., Pan, Z. K., Zhong, G. (2004). Activation of Raf/MEK/ERK/cPLA2 Signaling Pathway Is Essential for Chlamydial Acquisition of Host Glycerophospholipids. J. Biol. Chem. 279: 9409-9416 [Abstract] [Full Text]
Greene, W., Xiao, Y., Huang, Y., McClarty, G., Zhong, G. (2004). Chlamydia-Infected Cells Continue To Undergo Mitosis and Resist Induction of Apoptosis. Infect. Immun. 72: 451-460 [Abstract] [Full Text]
Gervassi, A. L., Probst, P., Stamm, W. E., Marrazzo, J., Grabstein, K. H., Alderson, M. R. (2003). Functional Characterization of Class Ia- and Non-Class Ia-Restricted Chlamydia-Reactive CD8+ T Cell Responses in Humans. J. Immunol. 171: 4278-4286 [Abstract] [Full Text]
Kincaid, E. Z., Ernst, J. D. (2003). Mycobacterium tuberculosis Exerts Gene-Selective Inhibition of Transcriptional Responses to IFN-{gamma} Without Inhibiting STAT1 Function. J. Immunol. 171: 2042-2049 [Abstract] [Full Text]
Belland, R. J., Zhong, G., Crane, D. D., Hogan, D., Sturdevant, D., Sharma, J., Beatty, W. L., Caldwell, H. D. (2003). Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc. Natl. Acad. Sci. USA 100: 8478-8483 [Abstract] [Full Text]
Wang, G., Burczynski, F., Hasinoff, B., Zhong, G. (2002). Infection of myocytes with chlamydiae. Microbiology 148: 3955-3959 [Abstract] [Full Text]
Rodel, J., Vogelsang, H., Prager, K., Hartmann, M., Schmidt, K.-H., Straube, E. (2002). Role of Interferon-Stimulated Gene Factor 3{gamma} and Beta Interferon in HLA Class I Enhancement in Synovial Fibroblasts upon Infection with Chlamydia trachomatis. Infect. Immun. 70: 6140-6146 [Abstract] [Full Text]
Ramos, M., Alvarez, I., Sesma, L., Logean, A., Rognan, D., Lopez de Castro, J. A. (2002). Molecular Mimicry of an HLA-B27-derived Ligand of Arthritis-linked Subtypes with Chlamydial Proteins. J. Biol. Chem. 277: 37573-37581 [Abstract] [Full Text]
Saren, A., Pascolo, S., Stevanovic, S., Dumrese, T., Puolakkainen, M., Sarvas, M., Rammensee, H.-G., Vuola, J. M. (2002). Identification of Chlamydia pneumoniae-Derived Mouse CD8 Epitopes. Infect. Immun. 70: 3336-3343 [Abstract] [Full Text]
Morrison, R. P., Caldwell, H. D. (2002). Immunity to Murine Chlamydial Genital Infection. Infect. Immun. 70: 2741-2751 [Full Text]
Fan, P., Dong, F., Huang, Y., Zhong, G. (2002). Chlamydia pneumoniae Secretion of a Protease-Like Activity Factor for Degrading Host Cell Transcription Factors Is Required for Major Histocompatibility Complex Antigen Expression. Infect. Immun. 70: 345-349 [Abstract] [Full Text]