Caspase Activation Is Required for Terminal Erythroid Differentiation

doi:10.1084/jem.193.2.247

Published online 16 January 2001.

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© The Rockefeller University Press, 0022-1007/2001/1/247/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 2, January 15, 2001 247-254

Brief Definitive Report

Caspase Activation Is Required for Terminal Erythroid Differentiation

Yael Zermati^a, Carmen Garrido^d, Sophie Amsellem^e, Serge Fishelson^e, Didier Bouscary^f, Françoise Valensi^b, Bruno Varet^a,c, Eric Solary^d, and Olivier Hermine^a,c
^a Centre National de la Recherche Scientifique Unité Mixte de Recherche 8603, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
^b Laboratoire d'Hématologie, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
^c Service d'Hématologie Clinique, Université René Descartes (Paris V), Institut Fédérative de Recherche Necker, 75743 Paris cedex 15, France
^d Institut National de la Santé et de la Recherche Médicale (INSERM) U517, Unité Fédérative de Recherche Medecine et Pharmacie, Dijon 21033, France
^e Laboratoire de Recherche d'Hémobiologie, Hôpital Cochin, 75014 Paris, France
^f INSERM U363, Hôpital Cochin, 75014 Paris, France

Correspondence to: Olivier Hermine, Service d'Hématologie Clinique and CNRS UMR 8603, Hôpital Necker, 149-161 rue de Sèvres, 75743 Paris cedex 15, France. Tel:33-1-44-49-52-83 Fax:33-1-44-49-52-80 E-mail:hermine{at}necker.fr.

The cysteine proteases known as caspases play a central rolein most apoptotic pathways. Here, we show that caspase inhibitorsarrest the maturation of human erythroid progenitors at earlystages of differentiation, before nucleus and chromatin condensation.Effector caspases such as caspase-3 are transiently activatedthrough the mitochondrial pathway during erythroblast differentiationand cleave proteins involved in nucleus integrity (lamin B)and chromatin condensation (acinus) without inducing cell deathand cleavage of GATA-1. These observations indicate a new functionfor caspases as key proteases in the process of erythroid differentiation.

Key Words: apoptosis, erythropoiesis, mitochondria, acinus, lamin B

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Fernando, P., Megeney, L. A. (2007). Is caspase-dependent apoptosis only cell differentiation taken to the extreme?. FASEB J. 21: 8-17 [Abstract] [Full Text]
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Somervaille, T. C. P., Linch, D. C., Khwaja, A. (2001). Growth factor withdrawal from primary human erythroid progenitors induces apoptosis through a pathway involving glycogen synthase kinase-3 and Bax. Blood 98: 1374-1381 [Abstract] [Full Text]